Yong Sun1,2, Di Chi1,2, Miaoxin Tan3, Kai Kang4, Maomao Zhang1,2, Xiangyuan Jin5, Xiaoping Leng6, Rui Cao1,2, Xianglan Liu2, Bo Yu1,2, Jian Wu1,2. 1. a Key Laboratories of Education Ministry for Myocardial Ischemia Mechanism and Treatment , Harbin Medical University , Harbin , China. 2. b Department of Cardiology , The Second Affiliated Hospital of Harbin Medical University , Harbin , China. 3. c Department of Cardiology , The First Hospital of Fangshan District , Beijing , china. 4. d Department of Cardial Surgery , The Second Affiliated Hospital of Harbin Medical University , Harbin , China. 5. e Department of Thoracic Surgery , The Third Affiliated Hospital of Harbin Medical University , Harbin , China. 6. f Department of Doppler Ultrasonic , The Second Affiliated Hospital of Harbin Medical University , Harbin , China.
Abstract
OBJECTIVE: Cardiosphere-derived cells (CDCs) improve cardiac function and attenuate remodeling in ischemic and non-ischemic cardiomyopathy, and are currently obtained through myocardial biopsy. However, there is not any study on whether functional CDCs may be obtained through cadaveric autopsy with similar benefits in non-ischemic cardiomyopathy. METHODS: Cardiac tissues from human or mouse cadavers were harvested, plated at 4°C, and removed at varying time points to culture human CDCs (CLH-EDCs) and mouse CDCs (CM-CDCs). The differentiation and paracrine effects of CDCs were also assessed. Furthermore, intramyocardial injection of cadaveric CM-CDCs was performed in an induced dilated cardiomyopathy (DCM) model. RESULTS: With the extension of post mortem hours, the number of CLH-EDCs and CM-CDCs harvested from autopsy specimens decreased. The expressions of von Willebrand factor (VWF) and smooth muscle actin (SMA) on CDCs were gradually reduced, however, cardiac troponin I (TNI) expression increased in the 24 h group compared to the 0 h group. CLH-EDCs were also found to have similar paracrine function in the 24 h group compared to 0 h group. 8 weeks after CM-CDCs transplantion to the injured heart, mean left ventricular ejection fraction increased in both 0 h (64.99 ± 3.4%) and 24 h (62.99 ± 2.8%) CM-CDCs-treated groups as compared to the PBS treated group (53.64 ± 5.6 cm), with a decrease in left ventricular internal diastolic diameter (0.29 ± 0.08 cm and 0.32 ± 0.04 cm in 0 h and 24 h groups, vs. 0.41 ± 0.05 cm in PBS group). CONCLUSION: CDCs from cadaveric autopsy are highly proliferative and differentiative, and may be used as a source for allograft transplantation, in order to decrease myocardial fibrosis, attenuate left ventricular remodeling, and improve heart function in doxorubicin-induced non-ischemic cardiomyopathy.
OBJECTIVE: Cardiosphere-derived cells (CDCs) improve cardiac function and attenuate remodeling in ischemic and non-ischemic cardiomyopathy, and are currently obtained through myocardial biopsy. However, there is not any study on whether functional CDCs may be obtained through cadaveric autopsy with similar benefits in non-ischemic cardiomyopathy. METHODS: Cardiac tissues from human or mouse cadavers were harvested, plated at 4°C, and removed at varying time points to culture humanCDCs (CLH-EDCs) and mouseCDCs (CM-CDCs). The differentiation and paracrine effects of CDCs were also assessed. Furthermore, intramyocardial injection of cadaveric CM-CDCs was performed in an induced dilated cardiomyopathy (DCM) model. RESULTS: With the extension of post mortem hours, the number of CLH-EDCs and CM-CDCs harvested from autopsy specimens decreased. The expressions of von Willebrand factor (VWF) and smooth muscle actin (SMA) on CDCs were gradually reduced, however, cardiac troponin I (TNI) expression increased in the 24 h group compared to the 0 h group. CLH-EDCs were also found to have similar paracrine function in the 24 h group compared to 0 h group. 8 weeks after CM-CDCs transplantion to the injured heart, mean left ventricular ejection fraction increased in both 0 h (64.99 ± 3.4%) and 24 h (62.99 ± 2.8%) CM-CDCs-treated groups as compared to the PBS treated group (53.64 ± 5.6 cm), with a decrease in left ventricular internal diastolic diameter (0.29 ± 0.08 cm and 0.32 ± 0.04 cm in 0 h and 24 h groups, vs. 0.41 ± 0.05 cm in PBS group). CONCLUSION:CDCs from cadaveric autopsy are highly proliferative and differentiative, and may be used as a source for allograft transplantation, in order to decrease myocardial fibrosis, attenuate left ventricular remodeling, and improve heart function in doxorubicin-induced non-ischemic cardiomyopathy.
Authors: Laura Erker; Hisaya Azuma; Andrew Y Lee; Changsheng Guo; Susan Orloff; Laura Eaton; Eric Benedetti; Bryan Jensen; Milton Finegold; Holger Willenbring; Markus Grompe Journal: Gastroenterology Date: 2010-06-02 Impact factor: 22.682
Authors: Vijay S Gorantla; Stefan Schneeberger; Linda R Moore; Vera S Donnenberg; Ludovic Zimmerlin; W P Andrew Lee; Albert D Donnenberg Journal: Cytotherapy Date: 2011-09-12 Impact factor: 5.414
Authors: Yao Liang Tang; Wuqiang Zhu; Min Cheng; Lijuan Chen; John Zhang; Tao Sun; Raj Kishore; M Ian Phillips; Douglas W Losordo; Gangjian Qin Journal: Circ Res Date: 2009-04-30 Impact factor: 17.367