| Literature DB >> 27057962 |
Nu Zhang1, Xu-Jie Zhang2, Yu-Long Song1, Xiao-Bing Lu1, Dan-Dan Chen3, Xiao-Qin Xia3, J Oriol Sunyer4, Yong-An Zhang5.
Abstract
Immunoglobulin light chain (IgL) is necessary for the assembly of an Ig molecule, which plays important roles in the immune response. IgL genes were identified in various teleost species, but the basic functions of different IgL isotypes and the preferential combination between IgL and IgH (Ig heavy chain) isotypes remain unclear. In the current study, by EST database searching and cDNA cloning in rainbow trout, 8 IgL sequences were obtained, which could be classified into the IgLκF, IgLκG, IgLσ and IgLλ isotypes, respectively. Trout IgL isotypes were highly expressed in the immune-related tissues, and participated in the immune responses in spleen and gut by stimulation with LPS and poly (I:C). The results of FACS and LC-MS/MS indicated that the IgLκG and IgLσ isotypes preferentially bonded with the heavy chains of IgM and IgT, respectively, in trout B cells and serum. In addition, the genomic organization of trout IgL isotypes and the utilization of recombination signal sequences were studied.Entities:
Keywords: Heavy chain; IgM; IgT; Light chain subisotype; Preferential combination; Rainbow trout
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Year: 2016 PMID： 27057962 DOI： 10.1016/j.dci.2016.04.001
Source DB: PubMed Journal: Dev Comp Immunol ISSN： 0145-305X Impact factor: 3.636