S Feng1, J D Song1. 1. Shu Feng, Jin-Dan Song, Key Laboratory of Cell Biology, Ministry of Public Health of China, China Medical University, Shenyang 110001, Liaonin Province, China.
Abstract
AIM: To study the relationship between β-glucuronidase and the invasiveness of human colorectal carcinoma cell lines. METHODS: Six colorectal carcinoma cell lines, including three well-differentiated (CX1, CCL187, and CCL229) and three poorly differentiated ones (CCL227, CCL228, and Clone A), were analyzed by Fischman's method to determine the concentration of β-glucuronidase in the medium. RESULTS: Low levels of β-glucuronidase (activity range: 1.29 to 1.96 μg/106 cells·h) were associated with poor invasiveness. This finding was in contrast to the elevated levels of the enzyme (2.46-3.37 μg/106·h) detected in the medium derived from the more aggressively invasive cells (CCL 227, CCL 228, Clone A, and CCL 229). CONCLUSION: Highly invasive colorectal carcinoma cells secreted higher levels of β-glucuronidase than the poorly invasive cells. Determination of secreted β-glucuronidase might represent a useful in vitro measurement tool to assess the invasiveness of colorectal carcinoma.
AIM: To study the relationship between β-glucuronidase and the invasiveness of humancolorectal carcinoma cell lines. METHODS: Six colorectal carcinoma cell lines, including three well-differentiated (CX1, CCL187, and CCL229) and three poorly differentiated ones (CCL227, CCL228, and Clone A), were analyzed by Fischman's method to determine the concentration of β-glucuronidase in the medium. RESULTS: Low levels of β-glucuronidase (activity range: 1.29 to 1.96 μg/106 cells·h) were associated with poor invasiveness. This finding was in contrast to the elevated levels of the enzyme (2.46-3.37 μg/106·h) detected in the medium derived from the more aggressively invasive cells (CCL 227, CCL 228, Clone A, and CCL 229). CONCLUSION: Highly invasive colorectal carcinoma cells secreted higher levels of β-glucuronidase than the poorly invasive cells. Determination of secreted β-glucuronidase might represent a useful in vitro measurement tool to assess the invasiveness of colorectal carcinoma.
Authors: G L Nicolson; M Nakajima; J L Herrmann; D G Menter; P G Cavanaugh; J S Park; D Marchetti Journal: J Neurooncol Date: 1994 Impact factor: 4.130
Authors: M H Zheng; Y Fan; A Panicker; A Smith; T Robertson; S Wysocki; P Robbins; J M Papadimitriou; D J Wood Journal: Am J Pathol Date: 1995-12 Impact factor: 4.307