Fu-Jun Li1, Zhi-Jian Cai2, Fei Yang2, Shou-De Zhang3, Ming Chen1. 1. a Department of Otolaryngology , the Second Affiliated Hospital, School of Medicine, Zhejiang University , Hangzhou , PR China ; 2. b Institute of Immunology, School of Medicine, Zhejiang University , Hangzhou , PR China ; 3. c Department of Otolaryngology , Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University , Hangzhou , PR China.
Abstract
CONCLUSION: The Th17 cell frequency in peripheral blood and levels of IL-17 showed significant differences between patients with laryngeal squamous cell carcinoma and those with vocal cords polyps. Serum levels of IL-17 were correlated with laryngocarcinoma staging. OBJECTIVES: To investigate associations among the frequency of Th17 cells, levels of IL-17, and laryngeal squamous cell carcinoma. METHOD: Eighty in-patients with laryngeal squamous cell carcinoma and 114 in-patients with polypus of the vocal cord were enrolled. Th17 cell frequencies in peripheral blood and serum levels of IL-17 were measured by flow cytometry and enzyme-linked immunosorbent assay, respectively. The tissue expression levels of IL-17 mRNA transcripts and protein were measured using quantitative RT-PCR or immunohistochemical detection, respectively. RESULTS: Th17 cell frequencies in peripheral blood and serum concentrations of IL-17 were significantly higher in patients with laryngocarcinoma compared with those in patients with polyps (p < 0.01 for both Th17 cells and IL-17 levels). Serum concentrations of IL-17 were significantly higher in patients with advanced laryngocarcinoma than in patients with early laryngocarcinoma (p < 0.01). The mRNA and protein levels of IL-17 were significantly higher in laryngocarcinoma tissues than in adjacent normal tissues (p < 0.01 for mRNA levels, p < 0.05 for protein levels).
CONCLUSION: The Th17 cell frequency in peripheral blood and levels of IL-17 showed significant differences between patients with laryngeal squamous cell carcinoma and those with vocal cords polyps. Serum levels of IL-17 were correlated with laryngocarcinoma staging. OBJECTIVES: To investigate associations among the frequency of Th17 cells, levels of IL-17, and laryngeal squamous cell carcinoma. METHOD: Eighty in-patients with laryngeal squamous cell carcinoma and 114 in-patients with polypus of the vocal cord were enrolled. Th17 cell frequencies in peripheral blood and serum levels of IL-17 were measured by flow cytometry and enzyme-linked immunosorbent assay, respectively. The tissue expression levels of IL-17 mRNA transcripts and protein were measured using quantitative RT-PCR or immunohistochemical detection, respectively. RESULTS: Th17 cell frequencies in peripheral blood and serum concentrations of IL-17 were significantly higher in patients with laryngocarcinoma compared with those in patients with polyps (p < 0.01 for both Th17 cells and IL-17 levels). Serum concentrations of IL-17 were significantly higher in patients with advanced laryngocarcinoma than in patients with early laryngocarcinoma (p < 0.01). The mRNA and protein levels of IL-17 were significantly higher in laryngocarcinoma tissues than in adjacent normal tissues (p < 0.01 for mRNA levels, p < 0.05 for protein levels).
Entities:
Keywords:
Prognosis; T-helper 17 cells; head and neck cancers; immunity and tumorigenesis; interleukin-17
Authors: Yang Song; Ming Yang; Hongjian Zhang; Yan Sun; Ye Tao; Huihui Li; Jing Zhang; Yuncheng Li; Jianming Yang Journal: J Immunol Res Date: 2020-12-18 Impact factor: 4.818