| Literature DB >> 27046270 |
Franziska Bühligen1, Rico Lucas1, Marcell Nikolausz1, Sabine Kleinsteuber2.
Abstract
We present a simple protocol for the cost- and time-efficient profiling of methanogens based on T-RFLP fingerprinting of mcrA amplicons. Sequence data were compiled from mesophilic lab-scale and full-scale biogas reactors operated under various conditions and fed with various substrates. The database facilitates the rapid identification of methanogens, thus reducing the need of cloning and sequencing.Keywords: Biogas; BstNI; Community fingerprinting; Methanogenic archaea; MwoI; mcrA
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Year: 2016 PMID: 27046270 DOI: 10.1016/j.anaerobe.2016.03.013
Source DB: PubMed Journal: Anaerobe ISSN: 1075-9964 Impact factor: 3.331