Rashmi Metgud1, Nidhi Khajuria2, Gayathri Ramesh3. 1. Professor and Head of Department, Department of Oral & Maxillofacial Pathology, Pacific Dental College , Udhaipur, India . 2. Senior Lecturer, Department of Department of Oral & Maxillofacial Pathology, Institute of Dental Sceinces , Jammu, India . 3. Reader, Department of Oral & Maxillofacial Pathology, Rama Dental College Hospital and Rescearch Center , Kanpur, Utter Predesh, India .
Abstract
INTRODUCTION: The ABO blood group system was the significant element for forensic serological examination of blood and body fluids in the past before the wide adaptation of DNA typing. A significant proportion of individuals (80%) are secretors, meaning that antigens present in the blood are also found in other body fluids such as saliva. Absorption inhibition is one such method that works by reducing strength of an antiserum based on type and amount of antigen present in the stains. AIM: To check the efficacy of identifying the blood group antigens in saliva and to know the secretor status using absorption inhibition method among southern Rajasthan population. MATERIALS AND METHODS: Blood and saliva samples were collected from 80 individuals comprising 20 individuals in each blood group. The absorption inhibition method was used to determine the blood group antigens in the saliva and then the results were correlated with the blood group of the collected blood sample. The compiled data was statistically analysed using chi-square test. RESULTS: Blood groups A & O revealed 100% secretor status for both males and females. While blood groups B and AB revealed 95% secretor status. CONCLUSION: Secretor status evaluation of the ABO blood group antigen in saliva using absorption inhibition method can be a useful tool in forensic examination.
INTRODUCTION: The ABO blood group system was the significant element for forensic serological examination of blood and body fluids in the past before the wide adaptation of DNA typing. A significant proportion of individuals (80%) are secretors, meaning that antigens present in the blood are also found in other body fluids such as saliva. Absorption inhibition is one such method that works by reducing strength of an antiserum based on type and amount of antigen present in the stains. AIM: To check the efficacy of identifying the blood group antigens in saliva and to know the secretor status using absorption inhibition method among southern Rajasthan population. MATERIALS AND METHODS: Blood and saliva samples were collected from 80 individuals comprising 20 individuals in each blood group. The absorption inhibition method was used to determine the blood group antigens in the saliva and then the results were correlated with the blood group of the collected blood sample. The compiled data was statistically analysed using chi-square test. RESULTS: Blood groups A & O revealed 100% secretor status for both males and females. While blood groups B and AB revealed 95% secretor status. CONCLUSION: Secretor status evaluation of the ABO blood group antigen in saliva using absorption inhibition method can be a useful tool in forensic examination.
Entities:
Keywords:
Blood stain; Body fluid; Forensic identification; Haemagglutination; Saliva analysis
Authors: Christopher J Day; Kate L Seib; Evgeny A Semchenko; Arun V Everest-Dass; Freda E-C Jen; Tsitsi D Mubaiwa Journal: mBio Date: 2019-07-09 Impact factor: 7.867
Authors: Marta Selma-Royo; Sonia González; Miguel Gueimonde; Melinda Chang; Annalee Fürst; Cecilia Martínez-Costa; Lars Bode; Maria Carmen Collado Journal: Mol Nutr Food Res Date: 2022-06-03 Impact factor: 6.575