Yogish Somayaji Tenkanidiyoor1, Vidya Vasudeva1, Shama Rao1, Damodara Gowda2, Chandrika Rao3, Ganesh Sanjeev4, Suchetha Kumari Nalilu5. 1. Research Scholar, Central Research Laboratory, K.S. Hegde Medical Academy, Nitte University , Deralakatte, Mangalore, Karnataka, India . 2. Assistant Professor, Department of Physiology, K.S. Hegde Medical Academy, Nitte University , Deralakatte, Mangalore, Karnataka, India . 3. Assistant Professor, Department of Pathology, K.S. Hegde Medical Academy, Nitte University , Deralakatte, Mangalore, Karnataka, India . 4. Radiation Physicist, Microtron centre, Mangalore University , Mangalore, Karnataka, India . 5. Professor, Department of Biochemistry, K.S. Hegde Medical Academy, Nitte University , Deralakatte, Mangalore, Karnataka, India .
Abstract
INTRODUCTION: Diallyl disulphide is an organo-sulphur compound which is present in garlic and responsible for the characteristic odor of garlic. It is known for its anticancer and invitro membrane stabilizing properties. AIM: The main aim was to evaluate the haematopoietic, antioxidant and membrane stabilizing property of diallyl disulfide in irradiated mice. MATERIALS AND METHODS: Mice were grouped into 6 groups as control, drug control, radiation control and drug pre-treatment groups (i.e. drug administration + radiation group) The mice were fed orally for 15 consecutive days and on the 15(th) day, one hour after drug administration, the mice were irradiated with 6Gy electron beam radiation. The changes in blood cell count, total antioxidant levels, malondialdehyde and reduced glutathione levels were determined. The immunomodulatory response of DADS to the radiological effects was determined by the estimation of IL-6 levels. RESULTS: A significant improvement in pre-drug treatment group when compared to control groups in the haemoglobin, red blood cell count, white blood cell count, haematocrit and platelet counts was observed. There is an increased level of interleukin-6 in the drug treated groups compared to the radiation control. An increase in the malondialdehyde levels and decrease in the glutathione levels in the irradiated group indicate increased lipid peroxidation and oxidative stress, whereas, there is a significant reduction in the malondialdehyde levels and increased glutathione levels in the drug pre-treatment groups showing membrane stabilization. CONCLUSION: Thus DADS proves to be an effective haematopoietic and antioxidative agent to counter radiation induced haematopoietic suppression and oxidative stress.
INTRODUCTION:Diallyl disulphide is an organo-sulphur compound which is present in garlic and responsible for the characteristic odor of garlic. It is known for its anticancer and invitro membrane stabilizing properties. AIM: The main aim was to evaluate the haematopoietic, antioxidant and membrane stabilizing property of diallyl disulfide in irradiated mice. MATERIALS AND METHODS:Mice were grouped into 6 groups as control, drug control, radiation control and drug pre-treatment groups (i.e. drug administration + radiation group) The mice were fed orally for 15 consecutive days and on the 15(th) day, one hour after drug administration, the mice were irradiated with 6Gy electron beam radiation. The changes in blood cell count, total antioxidant levels, malondialdehyde and reduced glutathione levels were determined. The immunomodulatory response of DADS to the radiological effects was determined by the estimation of IL-6 levels. RESULTS: A significant improvement in pre-drug treatment group when compared to control groups in the haemoglobin, red blood cell count, white blood cell count, haematocrit and platelet counts was observed. There is an increased level of interleukin-6 in the drug treated groups compared to the radiation control. An increase in the malondialdehyde levels and decrease in the glutathione levels in the irradiated group indicate increased lipid peroxidation and oxidative stress, whereas, there is a significant reduction in the malondialdehyde levels and increased glutathione levels in the drug pre-treatment groups showing membrane stabilization. CONCLUSION: Thus DADS proves to be an effective haematopoietic and antioxidative agent to counter radiation induced haematopoietic suppression and oxidative stress.
Entities:
Keywords:
Electron beam radiation; Interleukin-6; Membrane stabilization; Sulphydryl protection
Authors: F Herodin; J C Mestries; D Janodet; S Martin; J Mathieu; M P Gascon; M O Pernin; A Ythier Journal: Blood Date: 1992-08-01 Impact factor: 22.113