Seyed-Mostafa Hosseini-Zijoud1, Seyed Alireza Ebadi2, Mohammad Taghi Goodarzi3, Mehdi Hedayati4, Roghayeh Abbasalipourkabir5, Mohammad Parsa Mahjoob6, Jalal Poorolajal7, Fabio Zicker8, Nasrin Sheikh3. 1. Ph.D. Candidate in Clinical Biochemistry, Department of Biochemistry, School of Medicine, Hamadan University of Medical Sciences , Hamadan, Iran . 2. Associate Professor of Endocinology, Department of Internal Medicine, Faculty of Medicine, Shahid Beheshti University of Medical Sciences , Tehran, Iran . 3. Professor of Clinical Biochemistry, Department of Biochemistry, School of Medicine, Hamadan University of Medical Sciences , Hamadan, Iran . 4. Associate Professor of Biochemistry, Cellular and Molecular Endocrine Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences , Tehran, Iran . 5. Assistant Professor, Department of Clinical Biochemistry, Department of Biochemistry, School of Medicine, Hamadan University of Medical Sciences , Hamadan, Iran . 6. Assistant Professor, Department of Cardiology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences , Tehran, Iran . 7. Associate Professor of Epidemiology, Research Center for Health Sciences and Department of Epidemiology, School of Public Heath, Hamadan University of Medical Sciences , Hamadan, Iran . 8. Professor of Epidemiology, Center for Technological Development in Health, Oswaldo Cruz Foundation , Rio de Janeiro, Brazil .
Abstract
INTRODUCTION: Oxidative stress or oxidant/antioxidant imbalance has a crucial role in the pathogenesis of some diseases like cancer. Medullary thyroid carcinoma (MTC) originates in the thyroid parafollicular cells and includes 3-4% of the malignant neoplasms that have an effect on this gland. The aetiology of MTC has not been clarified. However, oxidative stress may be one of the factors involved. AIM: The aim of the current study was to evaluate the antioxidant enzyme activity of catalase (CAT), Glutathione (GSH), total antioxidant capacity (TAC) and the levels of the lipid peroxidation product malondialdehyde (MDA) in blood samples of MTC patients as compared to healthy controls. MATERIALS AND METHODS: A case-control study was designed enrolling patients with confirmed MTC diagnosis and age-and sex group matched healthy volunteers referred to the clinic of the Research Institute for Endocrine Sciences, Tehran, Iran from April 2013 to July 2015. Fasting blood samples were taken for study. Catalase, GSH, MDA and TAC levels were measured by colorimetry using commercial kits (ZellBio GmbH, Germany). Data were analysed using SPSS 17 software, comparing mean±SD through t-test and difference between proportions through chi-square. RESULTS: No statistical difference was observed in the demographic characteristic between cases and controls. The final MTC group included 40 males and 45 females with a mean age of 30±12.9 year, and the control group 40 males and 47 females, with a mean age of 31.2±12.3 year. Anthropometric parameters, dietary and thyroid hormones levels (T3, T4 and TSH) were similar. Serum TAC (p=0.015), GSH (p=0.029) and CAT (p<0.001) levels were found to be significantly lower in the MTC patients, while serum MDA levels were significantly higher in MTC patients than controls (p<0.001). CONCLUSION: These preliminary findings suggest that oxidant/antioxidant imbalance may be associated with or possibly indicate an increased risk to medullary thyroid carcinoma. Further studies are needed to explore these findings.
INTRODUCTION: Oxidative stress or oxidant/antioxidant imbalance has a crucial role in the pathogenesis of some diseases like cancer. Medullary thyroid carcinoma (MTC) originates in the thyroid parafollicular cells and includes 3-4% of the malignant neoplasms that have an effect on this gland. The aetiology of MTC has not been clarified. However, oxidative stress may be one of the factors involved. AIM: The aim of the current study was to evaluate the antioxidant enzyme activity of catalase (CAT), Glutathione (GSH), total antioxidant capacity (TAC) and the levels of the lipid peroxidation product malondialdehyde (MDA) in blood samples of MTC patients as compared to healthy controls. MATERIALS AND METHODS: A case-control study was designed enrolling patients with confirmed MTC diagnosis and age-and sex group matched healthy volunteers referred to the clinic of the Research Institute for Endocrine Sciences, Tehran, Iran from April 2013 to July 2015. Fasting blood samples were taken for study. Catalase, GSH, MDA and TAC levels were measured by colorimetry using commercial kits (ZellBio GmbH, Germany). Data were analysed using SPSS 17 software, comparing mean±SD through t-test and difference between proportions through chi-square. RESULTS: No statistical difference was observed in the demographic characteristic between cases and controls. The final MTC group included 40 males and 45 females with a mean age of 30±12.9 year, and the control group 40 males and 47 females, with a mean age of 31.2±12.3 year. Anthropometric parameters, dietary and thyroid hormones levels (T3, T4 and TSH) were similar. Serum TAC (p=0.015), GSH (p=0.029) and CAT (p<0.001) levels were found to be significantly lower in the MTC patients, while serum MDA levels were significantly higher in MTC patients than controls (p<0.001). CONCLUSION: These preliminary findings suggest that oxidant/antioxidant imbalance may be associated with or possibly indicate an increased risk to medullary thyroid carcinoma. Further studies are needed to explore these findings.
Entities:
Keywords:
Catalase; Glutathione; Malondialdehyde; Total antioxidant capacity
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