Anxiong Long1, Shungao Ma2, Qian Li3, Na Lin4, Xia Zhan4, Shuaijun Lu5, Yuli Zhu6, Liansheng Jiang3, Longyi Tan7. 1. Clinical Laboratory Department, Baoshan Branch of Shanghai First People's Hospital, Shanghai, China; Clinical Laboratory Department, People's Hospital of Dali Bai Autonomous Prefecture, Dali, China. 2. Clinical Laboratory Department, People's Hospital of Dali Bai Autonomous Prefecture, Dali, China. 3. Clinical Laboratory Department, Baoshan Branch of Shanghai First People's Hospital, Shanghai, China. 4. Institute of Pediatrics, Xinhua Hospital, Shanghai, China. 5. Clinical Laboratory Department, Affiliated Hospital of Ningbo University, Ningbo, China. 6. Clinical Laboratory Department, Jiujiang First People's Hospital, Jiujiang, China. 7. Clinical Laboratory Department, Baoshan Branch of Shanghai First People's Hospital, Shanghai, China. Electronic address: longyitandoc@163.com.
Abstract
OBJECTIVE: To investigate whether serum levels of 19 eicosanoids are associated with pre-eclampsia. METHODS: A case-control study was performed using data for pregnant women with pre-eclampsia, normotensive pregnant women, and nonpregnant women, for all of whom serum samples had been collected at a hospital in Shanghai, China, between December 2012 and December 2013. Ultra-performance liquid chromatography-tandem mass spectrometry was used to measure the serum levels of 19 eicosanoids. RESULTS: Overall, 49 pregnant women with pre-eclampsia, 26 normotensive pregnant women, and 14 nonpregnant women were included. Women with pre-eclampsia had significantly higher serum levels of 11,12-epoxyeicosatrienoic acid (11,12-EET), the hydroxyeicosatetraenoic acids 5-HETE, 8-HETE, 12-HETE, and 15-HETE, and leukotriene B4 than did women with a normal pregnancy and nonpregnant women, both before and after the onset of pre-eclampsia (P<0.01 for all comparisons). Women with severe pre-eclampsia had significantly higher serum levels of 5-HETE, 15-HETE, and leukotriene B4 than did women with mild pre-eclampsia, women with a normal pregnancy, and nonpregnant women (P<0.01 for all comparisons). CONCLUSION: The eicosanoids 11,12-EET, 5-HETE, 8-HETE, 12-HETE, 15-HETE, and leukotriene B4 might play important parts in the occurrence and development of pre-eclampsia.
OBJECTIVE: To investigate whether serum levels of 19 eicosanoids are associated with pre-eclampsia. METHODS: A case-control study was performed using data for pregnant women with pre-eclampsia, normotensive pregnant women, and nonpregnant women, for all of whom serum samples had been collected at a hospital in Shanghai, China, between December 2012 and December 2013. Ultra-performance liquid chromatography-tandem mass spectrometry was used to measure the serum levels of 19 eicosanoids. RESULTS: Overall, 49 pregnant women with pre-eclampsia, 26 normotensive pregnant women, and 14 nonpregnant women were included. Women with pre-eclampsia had significantly higher serum levels of 11,12-epoxyeicosatrienoic acid (11,12-EET), the hydroxyeicosatetraenoic acids5-HETE, 8-HETE, 12-HETE, and 15-HETE, and leukotriene B4 than did women with a normal pregnancy and nonpregnant women, both before and after the onset of pre-eclampsia (P<0.01 for all comparisons). Women with severe pre-eclampsia had significantly higher serum levels of 5-HETE, 15-HETE, and leukotriene B4 than did women with mild pre-eclampsia, women with a normal pregnancy, and nonpregnant women (P<0.01 for all comparisons). CONCLUSION: The eicosanoids11,12-EET, 5-HETE, 8-HETE, 12-HETE, 15-HETE, and leukotriene B4 might play important parts in the occurrence and development of pre-eclampsia.
Authors: Selina T Somani; Maxwell Zeigler; Emily E Fay; Maggie Leahy; Bethanee Bermudez; Rheem A Totah; Mary F Hebert Journal: Life Sci Date: 2020-10-15 Impact factor: 5.037
Authors: Max T Aung; Youfei Yu; Kelly K Ferguson; David E Cantonwine; Lixia Zeng; Thomas F McElrath; Subramaniam Pennathur; Bhramar Mukherjee; John D Meeker Journal: Sci Rep Date: 2019-11-19 Impact factor: 4.379