| Literature DB >> 27038271 |
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Abstract
The reagents and methods for purification and use of the most commonly used denaturants, guanidine hydrochloride (guanidine-HCl) and urea, are described. Other protein denaturants and reagents used to fold proteins are briefly mentioned. Sulfhydryl reagents (reducing agents) and "oxido-shuffling" (or oxidative regeneration) systems are also described.Entities:
Keywords: guanidine hydrochloride; oxidative regeneration; protein denaturants; protein folding; sulfhydryl reagents; urea
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Year: 2016 PMID： 27038271 DOI： 10.1002/0471140864.psa03as84
Source DB: PubMed Journal: Curr Protoc Protein Sci ISSN： 1934-3655