Literature DB >> 27037075

Transformation of Schizosaccharomyces pombe: Lithium Acetate/ Dimethyl Sulfoxide Procedure.

Johanne M Murray1, Adam T Watson1, Antony M Carr1.   

Abstract

Transformation ofSchizosaccharomyces pombewith DNA requires the conditioning of cells to promote DNA uptake followed by cell growth under conditions that select and maintain the plasmid or integration event. The three main methodologies are electroporation, treatment with lithium cations, and transformation of protoplasts. The lithium acetate method described here is widely used because it is simple and reliable.
© 2016 Cold Spring Harbor Laboratory Press.

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Year:  2016        PMID： 27037075     DOI： 10.1101/pdb.prot090969

Source DB:  PubMed          Journal:  Cold Spring Harb Protoc        ISSN： 1559-6095


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