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Literature DB >> 27033070

Detecting PTEN and PI3K Signaling in Brain.

Abstract

The central nervous system is comprised of multiple cell types including neurons, glia, and other supporting cells that may differ dramatically in levels of signaling pathway activation. Immunohistochemistry in conjunction with drug interference are powerful tools that allow evaluation of signaling pathways in different cell types of the mouse central nervous system in vivo. Here we provide detailed protocols for immunohistochemistry to evaluate three essential components in the PI3K pathway in mouse brain: Pten, p-Akt, and p-4ebp1, and for rapamycin treatment to modulate mTOR signaling in vivo.

Entities: Chemical Disease Gene Species

Keywords: Immunohistochemistry; PI3K; PTEN; Rapamycin; p-4EBP1; p-AKT

Mesh：

Substances：

Year: 2016 PMID： 27033070 PMCID： PMC4857604 DOI： 10.1007/978-1-4939-3299-3_5

Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745

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References

18 in total

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Authors: Margaret Sperow; Raymond B Berry; Ildar T Bayazitov; Guo Zhu; Suzanne J Baker; Stanislav S Zakharenko
Journal: J Physiol Date: 2011-12-06 Impact factor: 5.182

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Authors: D D Sarbassov; David A Guertin; Siraj M Ali; David M Sabatini
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3. Pten deletion causes mTorc1-dependent ectopic neuroblast differentiation without causing uniform migration defects.

Authors: Guo Zhu; Lionel M L Chow; Ildar T Bayazitov; Yiai Tong; Richard J Gilbertson; Stanislav S Zakharenko; David J Solecki; Suzanne J Baker
Journal: Development Date: 2012-08-08 Impact factor: 6.868

4. Turnover of the active fraction of IRS1 involves raptor-mTOR- and S6K1-dependent serine phosphorylation in cell culture models of tuberous sclerosis.

Authors: O Jameel Shah; Tony Hunter
Journal: Mol Cell Biol Date: 2006-09 Impact factor: 4.272

Detecting PTEN and PI3K Signaling in Brain.

1. Phosphatase and tensin homologue (PTEN) regulates synaptic plasticity independently of its effect on neuronal morphology and migration.

2. Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex.

3. Pten deletion causes mTorc1-dependent ectopic neuroblast differentiation without causing uniform migration defects.

4. Turnover of the active fraction of IRS1 involves raptor-mTOR- and S6K1-dependent serine phosphorylation in cell culture models of tuberous sclerosis.

5. Deletion of Pten in mouse brain causes seizures, ataxia and defects in soma size resembling Lhermitte-Duclos disease.

6. Prolonged rapamycin treatment inhibits mTORC2 assembly and Akt/PKB.

7. Cell type specificity of PI3K signaling in Pdk1- and Pten-deficient brains.

Review 8. PTEN and the PI3-kinase pathway in cancer.

9. mTor is required for hypertrophy of Pten-deficient neuronal soma in vivo.

Review 10. Regulation of the mTOR complex 1 pathway by nutrients, growth factors, and stress.