Literature DB >> 27033009

A new formula to calculate activity of superoxide dismutase in indirect assays.

Chen Zhang1, Marieke E Bruins2, Zhi-Qiang Yang3, Shu-Tao Liu4, Ping-Fan Rao5.   

Abstract

To calculate superoxide dismutase (SOD) activity rapidly and accurately by indirect SOD assays, a formula based on the ratio of the catalytic speed of SOD to the reaction speed of the indicator with superoxide anion was deduced. The accuracy of this formula was compared with the conventional formula based on inhibition in five indirect SOD assays. The new formula was validated in nearly the entire SOD activity range, whereas the conventional formula was validated only during inhibition of 40-60%. This formula might also be used for the assays of other enzymes.
Copyright © 2016 Elsevier Inc. All rights reserved.

Entities:  

Keywords:  Cytochrome c; Epinephrine; Hydroxylamine hydrochloride; Nitroblue tetrazolium; SOD; WST

Mesh：

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Year:  2016        PMID： 27033009     DOI： 10.1016/j.ab.2016.03.014

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  7 in total

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