Jacob K Olson1, Terrence M Rager1, Jason B Navarro2, Lauren Mashburn-Warren2, Steven D Goodman2, Gail E Besner3. 1. Center for Perinatal Research, The Research Institute at Nationwide Children's Hospital, Department of Pediatric Surgery, Nationwide Children's Hospital, Columbus, OH. 2. Center for Microbial Pathogenesis, The Research Institute at Nationwide Children's Hospital, Columbus, OH. 3. Center for Perinatal Research, The Research Institute at Nationwide Children's Hospital, Department of Pediatric Surgery, Nationwide Children's Hospital, Columbus, OH. Electronic address: Gail.Besner@NationwideChildrens.org.
Abstract
BACKGROUND/ PURPOSE: Probiotics reduce the incidence of necrotizing enterocolitis (NEC) albeit only when administered at high frequency (at least daily). We have developed a novel probiotic delivery system in which probiotics are grown as a biofilm on microspheres, allowing enhanced efficacy with only a single treatment. METHODS: Neonatal rats were subjected to experimental NEC. Pups received a single enteral dose of: (1) vehicle only, (2) unloaded microspheres, (3) MRS (broth)-loaded microspheres, (4) Lactobacillus reuteri, (5) L. reuteri grown on unloaded microspheres, or (6) L. reuteri grown on MRS-loaded microspheres. Intestinal injury was graded histologically and intestinal permeability determined by serum levels of enterally administered fluorescein isothiocyanate-labeled dextran. RESULTS: 69% of untreated pups developed NEC, whereas 32% of pups treated with L. reuteri grown as a biofilm on unloaded microspheres (p=0.009) and 33% of pups treated with L. reuteri grown as a biofilm on MRS-loaded microspheres (p=0.005) developed NEC. No other group had a significant reduction in NEC. Furthermore, pups treated with L. reuteri grown as a biofilm had significantly reduced intestinal permeability. CONCLUSIONS: A single dose of Lactobacillus biofilm grown on biocompatible microspheres significantly reduces NEC incidence and severity. This novel probiotic delivery system may be beneficial in the prevention of NEC in the future.
BACKGROUND/ PURPOSE: Probiotics reduce the incidence of necrotizing enterocolitis (NEC) albeit only when administered at high frequency (at least daily). We have developed a novel probiotic delivery system in which probiotics are grown as a biofilm on microspheres, allowing enhanced efficacy with only a single treatment. METHODS: Neonatal rats were subjected to experimental NEC. Pups received a single enteral dose of: (1) vehicle only, (2) unloaded microspheres, (3) MRS (broth)-loaded microspheres, (4) Lactobacillus reuteri, (5) L. reuteri grown on unloaded microspheres, or (6) L. reuteri grown on MRS-loaded microspheres. Intestinal injury was graded histologically and intestinal permeability determined by serum levels of enterally administered fluorescein isothiocyanate-labeled dextran. RESULTS: 69% of untreated pups developed NEC, whereas 32% of pups treated with L. reuteri grown as a biofilm on unloaded microspheres (p=0.009) and 33% of pups treated with L. reuteri grown as a biofilm on MRS-loaded microspheres (p=0.005) developed NEC. No other group had a significant reduction in NEC. Furthermore, pups treated with L. reuteri grown as a biofilm had significantly reduced intestinal permeability. CONCLUSIONS: A single dose of Lactobacillus biofilm grown on biocompatible microspheres significantly reduces NEC incidence and severity. This novel probiotic delivery system may be beneficial in the prevention of NEC in the future.
Authors: Jacob K Olson; Jason B Navarro; Jacob M Allen; Christopher J McCulloh; Lauren Mashburn-Warren; Yijie Wang; Vanessa A Varaljay; Michael T Bailey; Steven D Goodman; Gail E Besner Journal: Am J Physiol Gastrointest Liver Physiol Date: 2018-05-31 Impact factor: 4.052
Authors: Mecklin V Ragan; Samantha J Wala; Steven D Goodman; Michael T Bailey; Gail E Besner Journal: Front Cell Infect Microbiol Date: 2022-06-28 Impact factor: 6.073
Authors: Ilse H de Lange; Charlotte van Gorp; Laurens D Eeftinck Schattenkerk; Wim G van Gemert; Joep P M Derikx; Tim G A M Wolfs Journal: Nutrients Date: 2021-05-19 Impact factor: 5.717
Authors: Jason B Navarro; Lauren Mashburn-Warren; Lauren O Bakaletz; Michael T Bailey; Steven D Goodman Journal: Front Microbiol Date: 2017-03-27 Impact factor: 5.640
Authors: Yijie Wang; Robert M Jaggers; Pamela Mar; Jeffrey D Galley; Terri Shaffer; Adrian Rajab; Shivani Deshpande; Lauren Mashburn-Warren; John R Buzzo; Steven D Goodman; Michael T Bailey; Gail E Besner Journal: Brain Behav Immun Health Date: 2021-04-06