OBJECTIVE: Vitamin D has been linked to the immune response modulation and the integrity of the intestinal mucosal barrier. Therefore, vitamin D might be involved in bacterial translocation related to HIV infection. Our major aim was to analyze the association between plasma levels of 25-hydroxy-vitamin D [25(OH)D] and bacterial 16S ribosomal DNA (bactDNA) in 120 HIV/hepatitis c virus (HCV) coinfected patients. DESIGN: Cross-sectional study. METHODS: Plasma 25(OH)D levels were quantified by enzyme immunoassay. The vitamin D status was defined as deficient (<25 nmol/l), insufficient (25-74 nmol/l), and optimal (≥75 nmol/l) plasma levels. Plasma bactDNA levels were measured by quantitative real-time PCR. For bactDNA levels the cutoffs used were as follows: low [<p25th (46 copies/μl)], moderate [p25th to p50th (78 copies/μl)], high [p50th to p75th (159 copies/μl)], and very high (>p75th). RESULTS: Eighteen (15%) patients had 25(OH)D deficiency, 93 (77.5%) had insufficiency and nine (7.5%) had 25(OH)D optimal values. The bactDNA levels were lower in patients with 25(OH)D at least 75 nmol/l [37 copies/μl] than in patients with 25(OH)D insufficiency [84.2 copies/μl; P = 0.042]. Conversely, low bactDNA levels (<p25th) were found in 66.7% of patients with 25(OH)D optimal levels, whereas bactDNA levels above p25th were found only in 11.1% of them (P = 0.029). The plasma 25(OH)D not less than 75 nmol/l was associated with low bactDNA levels (<p25th) [adjusted OR = 8.13 (95% confidence interval = 1.82; 36.67); P = 0.006)]. The patients with optimal vitamin D status [25(OH)D ≥75 nmol/l] had lower plasma levels of CCL7 (P = 0.047) and basic fibroblast growth factor (P = 0.042). CONCLUSION: The optimal vitamin D status was associated with low bacterial translocation and inflammation in HIV/HCV coinfected patients.
OBJECTIVE:Vitamin D has been linked to the immune response modulation and the integrity of the intestinal mucosal barrier. Therefore, vitamin D might be involved in bacterial translocation related to HIV infection. Our major aim was to analyze the association between plasma levels of 25-hydroxy-vitamin D [25(OH)D] and bacterial 16S ribosomal DNA (bactDNA) in 120 HIV/hepatitis c virus (HCV) coinfectedpatients. DESIGN: Cross-sectional study. METHODS: Plasma 25(OH)D levels were quantified by enzyme immunoassay. The vitamin D status was defined as deficient (<25 nmol/l), insufficient (25-74 nmol/l), and optimal (≥75 nmol/l) plasma levels. Plasma bactDNA levels were measured by quantitative real-time PCR. For bactDNA levels the cutoffs used were as follows: low [<p25th (46 copies/μl)], moderate [p25th to p50th (78 copies/μl)], high [p50th to p75th (159 copies/μl)], and very high (>p75th). RESULTS: Eighteen (15%) patients had 25(OH)D deficiency, 93 (77.5%) had insufficiency and nine (7.5%) had 25(OH)D optimal values. The bactDNA levels were lower in patients with 25(OH)D at least 75 nmol/l [37 copies/μl] than in patients with 25(OH)Dinsufficiency [84.2 copies/μl; P = 0.042]. Conversely, low bactDNA levels (<p25th) were found in 66.7% of patients with 25(OH)D optimal levels, whereas bactDNA levels above p25th were found only in 11.1% of them (P = 0.029). The plasma 25(OH)D not less than 75 nmol/l was associated with low bactDNA levels (<p25th) [adjusted OR = 8.13 (95% confidence interval = 1.82; 36.67); P = 0.006)]. The patients with optimal vitamin D status [25(OH)D ≥75 nmol/l] had lower plasma levels of CCL7 (P = 0.047) and basic fibroblast growth factor (P = 0.042). CONCLUSION: The optimal vitamin D status was associated with low bacterial translocation and inflammation in HIV/HCV coinfectedpatients.
Authors: Carol L Vinton; Carly E Starke; Alexandra M Ortiz; Stephen H Lai; Jacob K Flynn; Ornella Sortino; Kenneth Knox; Irini Sereti; Jason M Brenchley Journal: Pathog Immun Date: 2020-04-24