Panpan Wang1,2, Ya Li2, Hang Xiao3, Yonghui Shi1,2, Guo-Wei Le1,2, Jin Sun4,5. 1. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, PR China. 2. Food Nutrition and Functional Factors Research Center, School of Food Science and Technology, Jiangnan University, Wuxi, PR China. 3. Department of Food Science, University of Massachusetts, Amherst, MA, USA. 4. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, PR China. sunj@jiangnan.edu.cn. 5. Food Nutrition and Functional Factors Research Center, School of Food Science and Technology, Jiangnan University, Wuxi, PR China. sunj@jiangnan.edu.cn.
Abstract
SCOPE: We previously reported that specific Lactobacillus reuteri colonized within mouse Peyer's patches (PP) effectively prevented high fat diet induced obesity and low-grade chronic inflammation. We further investigated the role of PP Lactobacillus reuteri on sIgA production in rats in this study. METHODS AND RESULTS: Lactobacilli were isolated from rat PP. All isolates were L. reuteri and belonged to three phenotypes according to amplified fragment length polymorphism analysis. Typical strains of two main clusters, PP1 and PP2, were used to treat control and vitamin A deficient (VAD) rats, respectively. The feeding of PP1 and PP2 affected sIgA and Lactobacillus diversity by strain-specific manner. Free sIgA was significantly increased by PP1 (p = 0.069) and PP2 (p < 0.05) in the control rats but not in the VAD rats. Only PP1 significantly changed PP Lactobacillus diversity in the control rats (p < 0.05). However, PP2 specifically changed ileal Lactobacillus diversity in both control and VAD rats. Fecal sIgA was correlated with PP Lactobacillus diversity (R(2) = 0.7958, p = 0.011). CONCLUSION: Modulation of sIgA production by PP L. reuteri of rat is dependent on vitamin A and change of Lactobacillus diversity in PP.
SCOPE: We previously reported that specific Lactobacillus reuteri colonized within mouse Peyer's patches (PP) effectively prevented high fat diet induced obesity and low-grade chronic inflammation. We further investigated the role of PP Lactobacillus reuteri on sIgA production in rats in this study. METHODS AND RESULTS: Lactobacilli were isolated from rat PP. All isolates were L. reuteri and belonged to three phenotypes according to amplified fragment length polymorphism analysis. Typical strains of two main clusters, PP1 and PP2, were used to treat control and vitamin A deficient (VAD) rats, respectively. The feeding of PP1 and PP2 affected sIgA and Lactobacillus diversity by strain-specific manner. Free sIgA was significantly increased by PP1 (p = 0.069) and PP2 (p < 0.05) in the control rats but not in the VAD rats. Only PP1 significantly changed PP Lactobacillus diversity in the control rats (p < 0.05). However, PP2 specifically changed ileal Lactobacillus diversity in both control and VAD rats. Fecal sIgA was correlated with PP Lactobacillus diversity (R(2) = 0.7958, p = 0.011). CONCLUSION: Modulation of sIgA production by PP L. reuteri of rat is dependent on vitamin A and change of Lactobacillus diversity in PP.
Authors: Lisa Perruzza; Francesco Strati; Giorgio Gargari; Anna Maria D'Erchia; Bruno Fosso; Graziano Pesole; Simone Guglielmetti; Fabio Grassi Journal: Sci Rep Date: 2019-06-27 Impact factor: 4.379