Dinh Van Nguyen1,2,3, Christopher Vidal1, Jamma Li1, Richard B Fulton1, Suran L Fernando1,2. 1. ImmunoRheumatology Laboratory, Pathology North - Northern Sydney, St Leonards, Australia. 2. Sydney Medical School - Northern, University of Sydney, Sydney, Australia. 3. Department of Allergy & Clinical Immunology, Hanoi Medical University, Hanoi, Vietnam.
Abstract
AIM: In prevention of allopurinol and abacavir hypersensitivity, screening HLA-B*58:01/57:01 has been highly recommended prior to commencing these therapies. Therefore, we aimed at developing and validating a rapid and robust screening method for HLA-B*58:01/57:01. MATERIALS & METHODS: Real-time polymerase chain reaction with TaqMan probes was employed to detect HLA-B*58:01/57:01. RESULTS: The newly developed assay has the sensitivity of 100% (95% CI: 79.4-100.0%), the specificity of 98.8% (95% CI: 93.6-99.9%), the positive predictive value of 94.1% (95% CI: 71.3-99.9%) and the negative predictive value of 100.0% (95% CI: 95.7-100.0%). The lowest limit of detection is 0.04 ng/µl of DNA. CONCLUSION: The present method is a rapid and robust assay that is appropriate for screening of HLA-B*58:01/*57:01 alleles.
AIM: In prevention of allopurinol and abacavirhypersensitivity, screening HLA-B*58:01/57:01 has been highly recommended prior to commencing these therapies. Therefore, we aimed at developing and validating a rapid and robust screening method for HLA-B*58:01/57:01. MATERIALS & METHODS: Real-time polymerase chain reaction with TaqMan probes was employed to detect HLA-B*58:01/57:01. RESULTS: The newly developed assay has the sensitivity of 100% (95% CI: 79.4-100.0%), the specificity of 98.8% (95% CI: 93.6-99.9%), the positive predictive value of 94.1% (95% CI: 71.3-99.9%) and the negative predictive value of 100.0% (95% CI: 95.7-100.0%). The lowest limit of detection is 0.04 ng/µl of DNA. CONCLUSION: The present method is a rapid and robust assay that is appropriate for screening of HLA-B*58:01/*57:01 alleles.
Authors: Jeffrey A Sparks; Medha Barbhaiya; Elizabeth W Karlson; Susan Y Ritter; Soumya Raychaudhuri; Cassandra C Corrigan; Fengxin Lu; Jacob Selhub; Daniel I Chasman; Nina P Paynter; Paul M Ridker; Daniel H Solomon Journal: Semin Arthritis Rheum Date: 2017-02-10 Impact factor: 5.532
Authors: Dinh Van Nguyen; Christopher Vida; Hieu Chi Chu; Richard Fulton; Jamma Li; Suran L Fernando Journal: Allergy Asthma Immunol Res Date: 2017-01 Impact factor: 5.764