| Literature DB >> 27023643 |
Yang Cao, Xiaoxi Zhou, Xiaoyuan Huang, Qinlu Li, Lili Gao, Lijun Jiang, Mei Huang, Jianfeng Zhou.
Abstract
Entities:
Year: 2016 PMID: 27023643 PMCID: PMC4811421 DOI: 10.1371/journal.pone.0152830
Source DB: PubMed Journal: PLoS One ISSN: 1932-6203 Impact factor: 3.240
Fig 5Effect of Tim-3 inhibition on Hela cell migration and invasion in vitro.
(A) Cell migration capability was determined with a wound healing assay. Photographs were taken immediately (0 h), at 24 h and 48 h after wounding. (B) Quantification of wound closure. The data present the mean distance of cell migration to the wound area at 24 h and 48 h after wounding in three independent wound sites per group. (C) The ability of the cells to invade Matrigel was analyzed by the transwell invasion assay through a gel matrix. Hela cells were either infected with ADV-GFP or with ADV-antisense Tim-3, After 10 h viable invasive cells were fixed and counted. Values and error bars shown in this graph represent the averages and standard deviations respectively, of three independent experiments. (D) Representative images of the transwell invasion assay.