| Literature DB >> 27021397 |
M A Vladimirsky1, L K Shipina1, E S Makeeva1, Y S Alyapkina1, A Y Mikheev2, V N Morozov3.
Abstract
A simple inexpensive technique for collection of airborne biomarkers of nosocomial infections is described. Biomarkers were collected on water-soluble electrospun nanofilters attached to a household vacuum cleaner from 6-10m(3) of air in 10-15min within several wards of a tuberculosis clinic. Filters were then dissolved in water and tested for the presence of the IS6110 and regX3 genes of Mycobacterium tuberculosis (MTB) using real-time polymerase chain reaction. It was demonstrated that trace amounts of airborne MTB DNA (>3gene copies/m(3)) were always present in the air and on the surfaces in the wards occupied with tuberculosis patients having positive smear tests.Entities:
Keywords: Aerosol; Mycobacteria detection; Polymerase chain reaction; Water-soluble filters
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Year: 2016 PMID： 27021397 DOI： 10.1016/j.jhin.2016.02.009
Source DB: PubMed Journal: J Hosp Infect ISSN： 0195-6701 Impact factor: 3.926