| Literature DB >> 27021100 |
E V Karaduleva1, E K Mubarakshina2, M G Sharapov2, A E Volkova2, O Yu Pimenov2,3, V K Ravin2, Yu M Kokoz2,3, V I Novoselov2.
Abstract
Antioxidant properties of recombinant peroxiredoxin-6 and chimeric protein PSH combining peroxidase and superoxide dismutase activities were studied on the model of retrograde perfusion of isolated rat heart under conditions of H2O2-induced oxidative stress. The exogenous antioxidant proteins exhibited cardioprotective properties manifested in heart rate normalization, maintenance of contractile activity of the myocardium, and prevention of H2O2-induced LPO in oxidative stress. Localization of peroxiredoxin-6 and PSH in the cardiac tissue was determined and myocardial structures most effectively protected by the antioxidant enzymes from ischemia/reperfusion-induced damages were identified. The results suggest that modified peroxiredoxins are promising components of perfusion media for preservation of isolated organs.Entities:
Keywords: oxidative stress; peroxiredoxins; retrograde perfusion of the heart
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Year: 2016 PMID： 27021100 DOI： 10.1007/s10517-016-3237-1
Source DB: PubMed Journal: Bull Exp Biol Med ISSN： 0007-4888 Impact factor: 0.804