Literature DB >> 27019354

Correction: Ubiquitin B in Cervical Cancer: Critical for the Maintenance of Cancer Stem-Like Cell Characters.

Yuan Tian, Wencheng Ding, Yingying Wang, Teng Ji, Shujuan Sun, Qingqing Mo, Pingbo Chen, Yong Fang, Jia Liu, Beibei Wang, Jianfeng Zhou, Ding Ma, Peng Wu.   

Abstract

Entities:  

Year:  2016        PMID: 27019354      PMCID: PMC4809553          DOI: 10.1371/journal.pone.0152813

Source DB:  PubMed          Journal:  PLoS One        ISSN: 1932-6203            Impact factor:   3.240


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The authors would like to correct Fig 1, as errors were introduced in the preparation of this figure for publication. In Fig 1C, the panels for HeLa at 7 days and at 10 days appear to be identical. The authors have provided a corrected version of Fig 1 here.
Fig 1

The increasing effect of colony formation and drug-resistance in HeLa/TSA.

A, HeLa cells were treated with 500 nM TSA for the indicated times and subjected to analysis the mRNA level of UbB, UBC, UbA52 and UbA80 by real-time PCR and the corresponding protein level by western blotting. B, HeLa cells were incubated with TSA for 24 hours at a dose ranging from 200 to 800 nM and subjected to analysis the mRNA level of UbB, UbC, UbA52 and UbA80 by real-time PCR and the corresponding protein level by western blotting. C, Upper panel: representative dishes of the colony forming assay at day 7, 10 and 14. Lower panel: numbers of colony formed in HeLa and HeLa/TSA at day 7, 10 and 14. The columns represent the average of three separate experiments; error bars, SD; *, p<0.05. D, HeLa/TSA cells were treated with TSA (1 μM), DDP (30 μM), and PTX (75 nM) for 48 h or UV, the apoptosis cells were quantified by Annexin V/PI staining and the flow cytometry analysis. The representative examples of the flow cytometry results were shown. E, Cells were treated as described in D, the average percentages of apoptosis cells were reported in the graphs. Values, mean percentages; error bars, SD; *, p < 0.05 (n = 3 replications).

The increasing effect of colony formation and drug-resistance in HeLa/TSA.

A, HeLa cells were treated with 500 nM TSA for the indicated times and subjected to analysis the mRNA level of UbB, UBC, UbA52 and UbA80 by real-time PCR and the corresponding protein level by western blotting. B, HeLa cells were incubated with TSA for 24 hours at a dose ranging from 200 to 800 nM and subjected to analysis the mRNA level of UbB, UbC, UbA52 and UbA80 by real-time PCR and the corresponding protein level by western blotting. C, Upper panel: representative dishes of the colony forming assay at day 7, 10 and 14. Lower panel: numbers of colony formed in HeLa and HeLa/TSA at day 7, 10 and 14. The columns represent the average of three separate experiments; error bars, SD; *, p<0.05. D, HeLa/TSA cells were treated with TSA (1 μM), DDP (30 μM), and PTX (75 nM) for 48 h or UV, the apoptosis cells were quantified by Annexin V/PI staining and the flow cytometry analysis. The representative examples of the flow cytometry results were shown. E, Cells were treated as described in D, the average percentages of apoptosis cells were reported in the graphs. Values, mean percentages; error bars, SD; *, p < 0.05 (n = 3 replications). The authors confirm that these changes do not alter their findings. The authors have provided the underlying images for all figures in the original article as Supporting Information.

Underlying images for all figures.

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  1 in total

1.  Ubiquitin B in cervical cancer: critical for the maintenance of cancer stem-like cell characters.

Authors:  Yuan Tian; Wencheng Ding; Yingying Wang; Teng Ji; Shujuan Sun; Qingqing Mo; Pingbo Chen; Yong Fang; Jia Liu; Beibei Wang; Jianfeng Zhou; Ding Ma; Peng Wu
Journal:  PLoS One       Date:  2013-12-18       Impact factor: 3.240

  1 in total
  1 in total

1.  Identification of genes and pathways in the synovia of women with osteoarthritis by bioinformatics analysis.

Authors:  Bobin Mi; Guohui Liu; Wu Zhou; Huijuan Lv; Yi Liu; Jing Liu
Journal:  Mol Med Rep       Date:  2018-01-15       Impact factor: 2.952

  1 in total

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