| Literature DB >> 27017510 |
Merve Caliskan1, Rasim Mogulkoc2, Abdulkerim Kasim Baltaci1, Esma Menevse3.
Abstract
The aim of present study was to determine the effect of 3',4'-dihydroxyflavonol (DiOHF) on lipid peroxidation in experimental brain ischemia-reperfusion in rats. Present study was performed on the 34 male Wistar-albino rats, weigth 350-400 g. Experiment groups were designed as 1-Sham; 2-Ischemia-reperfusion; animal were anesthesized and carotid arteried were clemped for 20 min and reperfusion (7 days). 3-DiOHF + Ischemia-reperfusion; DiOHF was given to animals as 10 mg/kg by intraperitoneal. 4- Ischemia + DiOHF + Reperfusion; 5- Ischemia-reperfusion + DiOHF. Blood samples and serebral cortex were analysed for malondyaldehyde (MDA), NO (nitric oxide), xanthine oxidase (XO), glutathione (GSH) and glutathione peroxidase (GPx). Blood MDA levels were significantly higher ischemia-reperfusion groups (P < 0.005). However, DiOHF inhibited MDA. Ischemia-reperfusion led to increased XO and NO but DiOHF supplementation reduced NO and XO. DiOHF increased GSH and GPx levels compared to ischemia-reperfusion group. All together, our present study showed that intraperitoneal DiOHF supplementation has protective effect on brain ischaemia-reperfusion injury in rat.Entities:
Keywords: 3′,4′-dihydroxyflavonol; Brain ischaemia–reperfusion; Free radicals; Rat
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Year: 2016 PMID： 27017510 DOI： 10.1007/s11064-016-1889-x
Source DB: PubMed Journal: Neurochem Res ISSN： 0364-3190 Impact factor: 3.996