Qiumei Yang1, Jie Yu1, Bing Yu1, Zhiqing Huang1, Keying Zhang1, Jun He1, Xiangbing Mao1, Ping Zheng1, Daiwen Chen2. 1. Animal Nutrition Institute, Sichuan Agricultural University, No. 46, Xinkang Road, Yaan, Sichuan, 625014, People's Republic of China. 2. Animal Nutrition Institute, Sichuan Agricultural University, No. 46, Xinkang Road, Yaan, Sichuan, 625014, People's Republic of China. chendw@sicau.edu.cn.
Abstract
INTRODUCTION: Different populations of satellite cells (SCs) have been identified, but their functional difference remains unclear. METHODS: We used cell-surface markers and paired box transcription factor 3 (Pax3)/paired box transcription factor 7 (Pax7) expression to separate SC populations. In addition, self-renewal, proliferation, and differentiation abilities of each population were analyzed. RESULTS: Pax3+ /Pax7- SCs exhibited higher proliferation ability characterized by forming clusters of myogenic colonies with more self-renewing cells after several passages, while Pax3- /Pax7+ SCs had faster differentiation. The myotubes derived from Pax3+ /Pax7- SCs tended to express slow-myosin heavy chain and exhibited rhythmic contraction, while myotubes originating from Pax3- /Pax7+ SCs primarily formed fast-myosin heavy chains characterized by transitory contraction. CONCLUSIONS: Pax3+ /Pax7- SCs exhibited the ability of long-term self-renewal and proliferation, whereas Pax3- /Pax7+ SCs demonstrated faster differentiation. Muscle Nerve 54: 943-951, 2016.
INTRODUCTION: Different populations of satellite cells (SCs) have been identified, but their functional difference remains unclear. METHODS: We used cell-surface markers and paired box transcription factor 3 (Pax3)/paired box transcription factor 7 (Pax7) expression to separate SC populations. In addition, self-renewal, proliferation, and differentiation abilities of each population were analyzed. RESULTS:Pax3+ /Pax7- SCs exhibited higher proliferation ability characterized by forming clusters of myogenic colonies with more self-renewing cells after several passages, while Pax3- /Pax7+ SCs had faster differentiation. The myotubes derived from Pax3+ /Pax7- SCs tended to express slow-myosin heavy chain and exhibited rhythmic contraction, while myotubes originating from Pax3- /Pax7+ SCs primarily formed fast-myosin heavy chains characterized by transitory contraction. CONCLUSIONS:Pax3+ /Pax7- SCs exhibited the ability of long-term self-renewal and proliferation, whereas Pax3- /Pax7+ SCs demonstrated faster differentiation. Muscle Nerve 54: 943-951, 2016.