Chunlin You1, Ryoichi Hamasuna2, Midori Ogawa3, Kazumasa Fukuda3, Toru Hachisuga4, Tetsuro Matsumoto2, Hatsumi Taniguchi3. 1. Department of Microbiology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan. Electronic address: youchunlin1982@hotmail.com. 2. Department of Urology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan. 3. Department of Microbiology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan. 4. Department of Obstetrics and Gynecology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.
Abstract
OBJECTIVES: To analyse the bacterial flora of urine from patients with male urethritis using the clone library method. METHODS: Urine specimens from patients with urethritis were used. The bacterial flora was analysed according to the 16S ribosomal RNA gene-based clone library method. In addition, Neisseria gonorrhoeae, Chlamydia trachomatis, Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma urealyticum or Ureaplasma parvum were detected by the conventional PCR methods (TMA or real-time PCR) and data from the clone library and conventional PCR methods were compared. RESULTS: Among 58 urine specimens, 38 were successfully analysed using the clone library method. From the specimens, 2427 clones were evaluated and 95 bacterial phylotypes were detected. N. gonorrhoeae was detected from 6 specimens and as the predominant bacterial species in 5 specimens. M. genitalium was detected from 5 specimens and as the predominant bacterial species in 3 specimens. C. trachomatis was detected from 15 specimens using the TMA method, but was detected from only 1 specimen using the clone library method. U. parvum was detected from only 2 specimens using the clone library method. In addition, Haemophilus influenzae and Neisseria meningitidis were also detected in 8 and 1 specimens, respectively. Gardnerella vaginalis, which is a potential pathogen for bacterial vaginitis in women, was detected in 10 specimens. CONCLUSIONS: The clone library method can detect the occupancy rate of each bacteria species among the bacterial flora and may be a new method for bacterial analyses in male urethritis.
OBJECTIVES: To analyse the bacterial flora of urine from patients with male urethritis using the clone library method. METHODS: Urine specimens from patients with urethritis were used. The bacterial flora was analysed according to the 16S ribosomal RNA gene-based clone library method. In addition, Neisseria gonorrhoeae, Chlamydia trachomatis, Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma urealyticum or Ureaplasma parvum were detected by the conventional PCR methods (TMA or real-time PCR) and data from the clone library and conventional PCR methods were compared. RESULTS: Among 58 urine specimens, 38 were successfully analysed using the clone library method. From the specimens, 2427 clones were evaluated and 95 bacterial phylotypes were detected. N. gonorrhoeae was detected from 6 specimens and as the predominant bacterial species in 5 specimens. M. genitalium was detected from 5 specimens and as the predominant bacterial species in 3 specimens. C. trachomatis was detected from 15 specimens using the TMA method, but was detected from only 1 specimen using the clone library method. U. parvum was detected from only 2 specimens using the clone library method. In addition, Haemophilus influenzae and Neisseria meningitidis were also detected in 8 and 1 specimens, respectively. Gardnerella vaginalis, which is a potential pathogen for bacterial vaginitis in women, was detected in 10 specimens. CONCLUSIONS: The clone library method can detect the occupancy rate of each bacteria species among the bacterial flora and may be a new method for bacterial analyses in male urethritis.
Authors: Maria Frølund; Arne Wikström; Peter Lidbrink; Waleed Abu Al-Soud; Niels Larsen; Christoffer Bugge Harder; Søren Johannes Sørensen; Jørgen Skov Jensen; Peter Ahrens Journal: PLoS One Date: 2018-07-27 Impact factor: 3.240
Authors: Moria A Borys; Sean E Hulsebosch; F Charles Mohr; Katherine D Watson; Jane E Sykes; Kenneth W Simpson; Jodi L Westropp Journal: J Vet Intern Med Date: 2018-12-05 Impact factor: 3.333