Draft Genome Sequence of Leuconostoc mesenteroides 406 Isolated from the Traditional Fermented Mare Milk Airag in Tuv Aimag, Mongolia.

Leuconostoc mesenteroides406 was isolated from the traditional fermented mare milk airag in Tuv Aimag, Mongolia. This strain produces an antilisterial bacteriocin. Here, we report the draft genome sequence of this organism.

GENOME ANNOUNCEMENT

Airag is the most popular traditional fermented milk in Mongolia and Inner Mongolia of China. It is made by fermentation of mainly mare milk with various species of lactic acid bacteria. Leuconostoc mesenteroides is a species of lactic acid bacteria frequently detected in airag (1). The genus Leuconostoc represents a group within the low-G+C-content Gram-positive bacteria. We previously isolated L. mesenteroides 406, which produces an antilisterial bacteriocin, from airag in Tuv Aimag, Mongolia (2).

The L. mesenteroides 406 genome was paired-end sequenced using Illumina’s MiSeq platform. Genomic libraries containing 600- to 1,000-bp inserts were constructed and sequenced, yielding 3,291,624 sequences that provided 376-fold coverage from both ends of the genomic clones. The sequence reads were assembled using Newbler version 2.8 (Roche), and the assembled genome consists of 69 contigs, with a total length of 2,004,312 bp. The draft genome of strain 406 contained 1,978 predicted protein-coding genes. Next, we compared the draft genome of strain 406 with that of L. mesenteroides 213M0 (accession no. BCMO01000000), which was isolated from another airag in Bulgan Aimag, Mongolia (3). Of the 1,978 protein-coding genes, 1,754 genes (89%) were shared by the two strains. The genome information of this species will be useful for further studies on food science and of its physiology, taxonomy, and ecology.

Nucleotide sequence accession numbers.

The draft genome sequence for L. mesenteroides 406 has been deposited in the DDBJ/GenBank/EMBL database under the accession numbers BCMP01000001 to BCMP01000069.