| Literature DB >> 27007877 |
Jian-Wei Zhu1, Si-Hong Liu1, Gui-Qiang Zhang1, Hui-Hua Xu1, Yu-Xuan Wang1, Yong Wu2, Ya-Min Liu1, Yan Wang1, Jun-Bo Liang1, Qi-Feng Guo3.
Abstract
A new Ru(II) complex [Ru(dmp)2(NMIP)](ClO4)2 (dmp = 2,9-dimethyl-1,10-phenanthroline, NMIP = 2'-(2″-nitro-3″,4″-methylenedioxyphenyl)imidazo[4',5'-f][1,10]-phenanthroline) was synthesized and characterized by elemental analysis, ESI-MS and (1)H NMR. The cytotoxic activity of the complex against MG-63, U2OS, HOS, and MC3T3-e1 cell lines was investigated by MTT method. The complex shows moderate cytotoxicity toward HOS (IC50 = 35.6 ± 2.6 µM) and MC3T3-e1 (IC50 = 41.6 ± 2.8 µM) cell lines. The morphological studies show that the complex can induce apoptosis in HOS cells and cause an increase of reactive oxygen species levels and a decrease in the mitochondrial membrane potential. The cell cycle distribution demonstrates that the complex inhibits the cell growth at S phase. Additionally, the antitumor activity in vivo reveals that the complex can induce a decrease in tumor weight.Entities:
Keywords: Apoptosis; Cell cycle arrest; Cytotoxicity in vitro and in vivo; Reactive oxygen species; Ruthenium(II) complex
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Year: 2016 PMID： 27007877 DOI： 10.1007/s00232-016-9889-y
Source DB: PubMed Journal: J Membr Biol ISSN： 0022-2631 Impact factor: 1.843