| Literature DB >> 27001760 |
Cécile Nabet1, Safiatou Doumbo1, Fakhri Jeddi1, Issaka Sagara1, Tommaso Manciulli1, Amadou Tapily1, Coralie L'Ollivier1, Abdoulaye Djimde1, Ogobara K Doumbo1, Renaud Piarroux2.
Abstract
We evaluated the use of positive malaria rapid diagnostic tests (mRDTs) to determine genetic diversity of Plasmodium falciparum in Mali. Genetic diversity was assessed via multiple loci variable number of tandem repeats analysis (MLVA). We performed DNA extraction from 104 positive and 30 negative used mRDTs that had been stored at ambient temperature for up to 14 months. Extracted DNA was analyzed via quantitative polymerase chain reaction (qPCR), and MLVA genotyping was then assessed on positive qPCR samples. Eighty-three of the positive mRDTs (83/104, 79.8%) and none of the negative mRDTs were confirmed P. falciparum positive via qPCR. We achieved complete genotyping of 90.4% (75/83) of the qPCR-positive samples. Genotyping revealed high genetic diversity among P. falciparum populations in Mali and an absence of population clustering. We show that mRDTs are useful to monitor P. falciparum genetic diversity and thereby can provide essential data to guide malaria control programs. © The American Society of Tropical Medicine and Hygiene.Entities:
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Year: 2016 PMID： 27001760 PMCID： PMC4889742 DOI： 10.4269/ajtmh.15-0832
Source DB: PubMed Journal: Am J Trop Med Hyg ISSN： 0002-9637 Impact factor: 2.345