Rewa Verma1, Jae Young Kim. 1. Department of Life Science, Gachon University, Seongnam, Republic of Korea.
Abstract
OBJECTIVE: To explore the effects of 1,25-dihydroxyvitamin D3 [1,25(OH)2D3], the active metabolite of vitamin D, on M1/M2 polarization of human microglia and the expression of Toll-like receptor 10 (TLR10) on these cells, which has been suggested to play an inhibitory role in inflammation previously. METHODS: Microglial HMO6 cells were treated with 1,25(OH)2D3, and mRNA or protein levels of M1 and M2 cytokines and TLR10 were examined. RESULTS: 1,25(OH)2D3 upregulated TLR10 in HMO6 cells at both mRNA and protein level. 1,25(OH)2D3 enhanced basal mRNA expression of M2 cytokines, such as IL-10 and CCL17, but did not affect the expression of M1 cytokines, including IL-12 and TNF-α. 1,25(OH)2D3 downregulated the lipopolysaccharide (LPS)-induced mRNA expression of M1 cytokines IL-12 and TNF-α. Concomitantly, it upregulated not only the M2 cytokines IL-10 and CCL17, but also TLR10 in microglial cells treated with LPS, in a concentration-dependent manner. CONCLUSIONS: Our results suggest that 1,25(OH)2D3 may exert anti-inflammatory action by facilitating the M2 polarization of human microglial cells.
OBJECTIVE: To explore the effects of 1,25-dihydroxyvitamin D3 [1,25(OH)2D3], the active metabolite of vitamin D, on M1/M2 polarization of human microglia and the expression of Toll-like receptor 10 (TLR10) on these cells, which has been suggested to play an inhibitory role in inflammation previously. METHODS: Microglial HMO6 cells were treated with 1,25(OH)2D3, and mRNA or protein levels of M1 and M2 cytokines and TLR10 were examined. RESULTS:1,25(OH)2D3 upregulated TLR10 in HMO6 cells at both mRNA and protein level. 1,25(OH)2D3 enhanced basal mRNA expression of M2 cytokines, such as IL-10 and CCL17, but did not affect the expression of M1 cytokines, including IL-12 and TNF-α. 1,25(OH)2D3 downregulated the lipopolysaccharide (LPS)-induced mRNA expression of M1 cytokines IL-12 and TNF-α. Concomitantly, it upregulated not only the M2 cytokines IL-10 and CCL17, but also TLR10 in microglial cells treated with LPS, in a concentration-dependent manner. CONCLUSIONS: Our results suggest that 1,25(OH)2D3 may exert anti-inflammatory action by facilitating the M2 polarization of human microglial cells.
Authors: Hyun Ah Kim; Andrea Perrelli; Alberto Ragni; Francesca Retta; T Michael De Silva; Christopher G Sobey; Saverio Francesco Retta Journal: Antioxidants (Basel) Date: 2020-04-17
Authors: Megan A Evans; Hyun Ah Kim; Yeong Hann Ling; Sandy Uong; Antony Vinh; T Michael De Silva; Thiruma V Arumugam; Andrew N Clarkson; Graeme R Zosky; Grant R Drummond; Brad R S Broughton; Christopher G Sobey Journal: Neuromolecular Med Date: 2018-02-23 Impact factor: 3.843