Hélène Cappy1, Paolo Giacobini2, Pascal Pigny3, Aude Bruyneel1, Maryse Leroy-Billiard1, Didier Dewailly1, Sophie Catteau-Jonard4. 1. Departments of Endocrine Gynaecology and Reproductive Medicine, Hôpital Jeanne-de-Flandre, CHRU de Lille, Faculty of Medicine, Université de Lille II, 59037 Lille, France. 2. Inserm U1172 Team 2, JPARC, Université de Lille II, 59045 Lille, France. 3. Laboratory of Biochemistry and Hormonology, Biology Center, CHRU de Lille, 59037 Lille, France. 4. Departments of Endocrine Gynaecology and Reproductive Medicine, Hôpital Jeanne-de-Flandre, CHRU de Lille, Faculty of Medicine, Université de Lille II, 59037 Lille, France; Inserm U1172 Team 2, JPARC, Université de Lille II, 59045 Lille, France. Electronic address: sophie.jonard@chru-lille.fr.
Abstract
OBJECTIVES: Low vitamin D serum level has been reported in women with polycystic ovary syndrome (PCOS) compared to controls. A few in vitro studies showed that the bioactive form of vitamin D is able to modulate the expression of the anti-Müllerian hormone (AMH) gene. However, in vivo studies failed to demonstrate clearly whether low vitamin D3 serum level is involved in the AMH excess of PCOS. This prospective study evaluates serum vitamin D3 and AMH levels in women with PCOS and in controls, before and after vitamin D supplementation. MATERIALS AND METHODS: Among vitamin D deficient patients, 23 patients with PCOS were compared to 27 women with normal ovarian reserve (NOR). The vitamin D deficient patients received a vitamin D supplementation according to the depth of their insufficiency. For the 23 patients with PCOS and the 27 controls, serum AMH assay and serum calciotropic hormone assays [25-hydroxyvitamin D (25[OH]D), 1,25 dihydroxyvitamin D (1,25[OH]2D) and parathyroid hormone (PTH)] were performed before and after supplementation. RESULTS: Serum 25(OH)D levels before treatment were statistically lower in PCOS women than in NOR patients (P<0.05), even after adjustment for BMI, age and AMH level, but not after adjustment for waist circumference measurement. No difference in the serum AMH levels before and after treatment was observed neither in PCOS patients nor in NOR patients. In both groups, 25(OH)D serum levels were not related to serum AMH levels, serum 1,25(OH)2D and serum PTH levels, before and after treatment. CONCLUSION: We found no evidence that serum calciotropic hormones are linked to circulating AMH levels, particularly in PCOS.
OBJECTIVES: Low vitamin D serum level has been reported in women with polycystic ovary syndrome (PCOS) compared to controls. A few in vitro studies showed that the bioactive form of vitamin D is able to modulate the expression of the anti-Müllerian hormone (AMH) gene. However, in vivo studies failed to demonstrate clearly whether low vitamin D3 serum level is involved in the AMH excess of PCOS. This prospective study evaluates serum vitamin D3 and AMH levels in women with PCOS and in controls, before and after vitamin D supplementation. MATERIALS AND METHODS: Among vitamin Ddeficientpatients, 23 patients with PCOS were compared to 27 women with normal ovarian reserve (NOR). The vitamin Ddeficientpatients received a vitamin D supplementation according to the depth of their insufficiency. For the 23 patients with PCOS and the 27 controls, serum AMH assay and serum calciotropic hormone assays [25-hydroxyvitamin D (25[OH]D), 1,25 dihydroxyvitamin D (1,25[OH]2D) and parathyroid hormone (PTH)] were performed before and after supplementation. RESULTS: Serum 25(OH)D levels before treatment were statistically lower in PCOSwomen than in NOR patients (P<0.05), even after adjustment for BMI, age and AMH level, but not after adjustment for waist circumference measurement. No difference in the serum AMH levels before and after treatment was observed neither in PCOSpatients nor in NOR patients. In both groups, 25(OH)D serum levels were not related to serum AMH levels, serum 1,25(OH)2D and serum PTH levels, before and after treatment. CONCLUSION: We found no evidence that serum calciotropic hormones are linked to circulating AMH levels, particularly in PCOS.
Authors: Engy Bakeer; Rasha Radwan; Ahmed El Mandoury; Abdullah Abd El Rahman; Mohamed Gad; Sahar Abd El Maksoud Journal: J Med Biochem Date: 2018-12-01 Impact factor: 3.402