Charlotte Peeters1, Eliza Depoorter2, Jessy Praet3, Peter Vandamme4. 1. Laboratory of Microbiology, Ghent University, K. L. Ledeganckstraat 35, 9000 Ghent, Belgium. Electronic address: charlotte.peeters@ugent.be. 2. Laboratory of Microbiology, Ghent University, K. L. Ledeganckstraat 35, 9000 Ghent, Belgium. Electronic address: eliza.depoorter@ugent.be. 3. Laboratory of Microbiology, Ghent University, K. L. Ledeganckstraat 35, 9000 Ghent, Belgium. Electronic address: jessy.praet@ugent.be. 4. Laboratory of Microbiology, Ghent University, K. L. Ledeganckstraat 35, 9000 Ghent, Belgium. Electronic address: peter.vandamme@ugent.be.
Abstract
BACKGROUND: While the epidemiology of Burkholderia cepacia complex (Bcc) bacteria in cystic fibrosis (CF) patients suggests that Burkholderia multivorans is acquired from environmental sources, this species has rarely been isolated from soil and water samples. METHODS: Multiple isolation strategies were applied to water and soil samples that were previously shown to be B. multivorans PCR positive. These included direct plating and liquid enrichment procedures and the use of selective media, acclimatizing recovery and co-cultivation with CF sputum. MALDI-TOF mass spectrometry and sequence analysis of 16S rRNA and housekeeping genes were used to identify all isolates. RESULTS: None of the approaches yielded B. multivorans isolates. Other Burkholderia species, several Gram-negative non-fermenting bacteria (including Cupriavidus, Inquilinus, Pandoraea, Pseudomonas and Stenotrophomonas) and rapidly growing mycobacteria (including Mycobacterium chelonae) were all isolated from water and soil samples. CONCLUSIONS: The use of Bcc isolation media yielded a surprisingly wide array of rare but often clinically relevant CF pathogens, confirming that soil and water are reservoirs of these infectious agents. Copyright Â
BACKGROUND: While the epidemiology of Burkholderia cepacia complex (Bcc) bacteria in cystic fibrosis (CF) patients suggests that Burkholderia multivorans is acquired from environmental sources, this species has rarely been isolated from soil and water samples. METHODS: Multiple isolation strategies were applied to water and soil samples that were previously shown to be B. multivorans PCR positive. These included direct plating and liquid enrichment procedures and the use of selective media, acclimatizing recovery and co-cultivation with CF sputum. MALDI-TOF mass spectrometry and sequence analysis of 16S rRNA and housekeeping genes were used to identify all isolates. RESULTS: None of the approaches yielded B. multivorans isolates. Other Burkholderia species, several Gram-negative non-fermenting bacteria (including Cupriavidus, Inquilinus, Pandoraea, Pseudomonas and Stenotrophomonas) and rapidly growing mycobacteria (including Mycobacterium chelonae) were all isolated from water and soil samples. CONCLUSIONS: The use of Bcc isolation media yielded a surprisingly wide array of rare but often clinically relevant CF pathogens, confirming that soil and water are reservoirs of these infectious agents. Copyright Â
Authors: Charlotte Peeters; Jan P Meier-Kolthoff; Bart Verheyde; Evie De Brandt; Vaughn S Cooper; Peter Vandamme Journal: Front Microbiol Date: 2016-06-08 Impact factor: 5.640
Authors: Erin P Price; Derek S Sarovich; Jessica R Webb; Carina M Hall; Sierra A Jaramillo; Jason W Sahl; Mirjam Kaestli; Mark Mayo; Glenda Harrington; Anthony L Baker; Lindsay C Sidak-Loftis; Erik W Settles; Madeline Lummis; James M Schupp; John D Gillece; Apichai Tuanyok; Jeffrey Warner; Joseph D Busch; Paul Keim; Bart J Currie; David M Wagner Journal: PLoS Negl Trop Dis Date: 2017-09-14
Authors: Cédric Lood; Charlotte Peeters; Quentin Lamy-Besnier; Jeroen Wagemans; Daniel De Vos; Marijke Proesmans; Jean-Paul Pirnay; Fedoua Echahidi; Denis Piérard; Matthieu Thimmesch; Anca Boeras; Katrien Lagrou; Evelien De Canck; Elke De Wachter; Vera van Noort; Rob Lavigne; Peter Vandamme Journal: PLoS Pathog Date: 2021-03-15 Impact factor: 6.823
Authors: Charlotte Peeters; Evelien De Canck; Margo Cnockaert; Evie De Brandt; Cindy Snauwaert; Bart Verheyde; Eliza Depoorter; Theodore Spilker; John J LiPuma; Peter Vandamme Journal: Front Microbiol Date: 2019-11-06 Impact factor: 5.640