Sabrina Schmitz1, Julia Henke2, Sabine Tacke3, Brian Guth4. 1. Department of Nonclinical Drug Safety, Biological Laboratory Service, Boehringer Ingelheim Pharma GmbH & Co. KG, Birkendorfer Str. 65, 88397 Biberach, Germany. Electronic address: sabrina.schmitz@boehringer-ingelheim.com. 2. Department of Nonclinical Drug Safety, Biological Laboratory Service, Boehringer Ingelheim Pharma GmbH & Co. KG, Birkendorfer Str. 65, 88397 Biberach, Germany. 3. Department of Veterinary Clinical Sciences, Clinic for Small Animal-Surgery, Justus-Liebig-University, Frankfurter Str. 108, 35392 Giessen, Germany. Electronic address: Sabine.P.Tacke@vetmed.uni-giessen.de. 4. Department of Drug Discovery Support, General Pharmacology, Boehringer Ingelheim Pharma GmbH & Co. KG, Birkendorfer Str. 65, 88397 Biberach, Germany; DST/NWU Preclinical Drug Development Platform, Faculty of Health Sciences, North-West University, Potchefstroom, South Africa. Electronic address: brian.guth@boehringer-ingelheim.com.
Abstract
INTRODUCTION: Guinea pigs (GPs) are a valuable cardiovascular pharmacology model. Implantation of a radio-telemetry system into GPs is, however, challenging and has been associated with a high failure rate in the past. We provide information on a novel procedure for implanting telemetry devices into GPs and we have measured the hemodynamics (arterial blood pressure, BP and heart rate, HR) and core body temperature (BT) in the 24h after surgery. METHODS: Male Hartley GPs (Crl:HA, 350-400g, 6.5weeks, n=16) were implanted with a radio transmitter abdominally and were then monitored continuously (HR, BP and BT) for 24h after surgery. RESULTS: 13 of 16 GPs (81%) survived the surgery. Surgery duration was 94min (min) (range: 76-112min) and anaesthesia duration was 131min (range: 107-158min). GPs lost body weight until 2days after surgery and then regained weight. Mean arterial BP increased from 33.7mmHg directly after surgery to 59.1mmHg after 24h. HR increased from 206bpm directly after surgery to 286bpm at 8h and fell to 251bpm at 24h after implantation. BT was 36°C directly after surgery, fell to 35.4°C until regaining of the righting reflex and then stabilized at 38.5°C after 24h. DISCUSSION: A high survival rate in telemetered GPs is possible. We achieved this through a procedure with minimal stress through habituation and planning, continuous warming during anaesthesia, an optimal anaesthetic and analgesic management, efficient surgical techniques and vitamin C supplementation.
INTRODUCTION:Guinea pigs (GPs) are a valuable cardiovascular pharmacology model. Implantation of a radio-telemetry system into GPs is, however, challenging and has been associated with a high failure rate in the past. We provide information on a novel procedure for implanting telemetry devices into GPs and we have measured the hemodynamics (arterial blood pressure, BP and heart rate, HR) and core body temperature (BT) in the 24h after surgery. METHODS: Male Hartley GPs (Crl:HA, 350-400g, 6.5weeks, n=16) were implanted with a radio transmitter abdominally and were then monitored continuously (HR, BP and BT) for 24h after surgery. RESULTS: 13 of 16 GPs (81%) survived the surgery. Surgery duration was 94min (min) (range: 76-112min) and anaesthesia duration was 131min (range: 107-158min). GPs lost body weight until 2days after surgery and then regained weight. Mean arterial BP increased from 33.7mmHg directly after surgery to 59.1mmHg after 24h. HR increased from 206bpm directly after surgery to 286bpm at 8h and fell to 251bpm at 24h after implantation. BT was 36°C directly after surgery, fell to 35.4°C until regaining of the righting reflex and then stabilized at 38.5°C after 24h. DISCUSSION: A high survival rate in telemetered GPs is possible. We achieved this through a procedure with minimal stress through habituation and planning, continuous warming during anaesthesia, an optimal anaesthetic and analgesic management, efficient surgical techniques and vitamin C supplementation.
Authors: Loren P Thompson; Ling Chen; Brian M Polster; Gerard Pinkas; Hong Song Journal: Am J Physiol Regul Integr Comp Physiol Date: 2018-10-26 Impact factor: 3.619
Authors: Pierre Morissette; Sebastian Polak; Anne Chain; Jin Zhai; John P Imredy; Mary Jo Wildey; Jeffrey Travis; Kevin Fitzgerald; Patrick Fanelli; Elisa Passini; Blanca Rodriguez; Frederick Sannajust; Christopher Regan Journal: Toxicol Appl Pharmacol Date: 2020-01-23 Impact factor: 4.219