Yikai Zhang1,2, Jing Jia3, Yi Ding1, Yongzheng Ma4, Peijin Shang1, Tianlong Liu1, Guangfei Hui2, Lin Wang5, Mingming Wang1, Zhihui Zhu1, Yuwen Li1, Aidong Wen1. 1. Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi'an, China. 2. Department of Pharmacy, Chinese PLA Shenyang General Hospital, Shenyang Military Area Command, Shenyang, China. 3. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China. 4. Department of Biochemistry and Molecular Biology, State Key Laboratory of Cancer Biology, Fourth Military Medical University, Xi'an, China. 5. Department of Pharmacy, No. 313 Hospital of Chinese PLA, Huludao, China.
Abstract
OBJECTIVES: The purpose of this study was to assess the gastroprotective properties of alpha-boswellic acid (α-BA), a pentacyclic triterpene compound from extracts of Frankincense. METHODS: The gastroprotection of α-BA was assessed with ethanol-induced gastric lesions model, by histopathological assessment and measuring gastric juice acidity (pH), gastric wall mucus (GWM), prostaglandins E2 (PGE-2), membrane lipids peroxidation (MDA), superoxide dismutase (SOD) activity, catalase (CAT) activity and amount of nitric oxide (NO). The gastroprotective effects of α-BA through the nuclear factor erythroid-2-related factor 2/heme oxygenase-1 (Nrf2/HO-1) anti-oxidative pathway were presented and measured by immunohistochemistry and Western blot. KEY FINDINGS: The results showed that α-BA reduced injuries associated with the administration of ethanol, gastric juice acidity and the formation of MDA and increased CAT activity and SOD activity and the level of NO and PGE-2 in a dose-depended manner. The expression of both Nrf2 and HO-1 was significantly increased in the group treated with 200 mg/kg α-BA, which suggested that activation of the Nrf2/HO-1 pathway might be critical in α-BA's prevention of gastric ulcers. CONCLUSIONS: These findings demonstrate that α-BA decreases oxidative stress and that the Nrf2/HO-1 pathway might play a role in the gastroprotective action of α-BA in ethanol-induced gastric injury in rats.
OBJECTIVES: The purpose of this study was to assess the gastroprotective properties of alpha-boswellic acid (α-BA), a pentacyclictriterpene compound from extracts of Frankincense. METHODS: The gastroprotection of α-BA was assessed with ethanol-induced gastric lesions model, by histopathological assessment and measuring gastric juice acidity (pH), gastric wall mucus (GWM), prostaglandins E2 (PGE-2), membrane lipids peroxidation (MDA), superoxide dismutase (SOD) activity, catalase (CAT) activity and amount of nitric oxide (NO). The gastroprotective effects of α-BA through the nuclear factor erythroid-2-related factor 2/heme oxygenase-1 (Nrf2/HO-1) anti-oxidative pathway were presented and measured by immunohistochemistry and Western blot. KEY FINDINGS: The results showed that α-BA reduced injuries associated with the administration of ethanol, gastric juice acidity and the formation of MDA and increased CAT activity and SOD activity and the level of NO and PGE-2 in a dose-depended manner. The expression of both Nrf2 and HO-1 was significantly increased in the group treated with 200 mg/kg α-BA, which suggested that activation of the Nrf2/HO-1 pathway might be critical in α-BA's prevention of gastric ulcers. CONCLUSIONS: These findings demonstrate that α-BA decreases oxidative stress and that the Nrf2/HO-1 pathway might play a role in the gastroprotective action of α-BA in ethanol-induced gastric injury in rats.
Authors: Mohammad Raish; Mudassar Shahid; Yousef A Bin Jardan; Mushtaq Ahmad Ansari; Khalid M Alkharfy; Abdul Ahad; Ibrahim Abdelsalam Abdelrahman; Ajaz Ahmad; Fahad I Al-Jenoobi Journal: Front Pharmacol Date: 2021-02-25 Impact factor: 5.810