Zahra Samadi Noshahr1, Mohammad Reza Shahraki2, Hassan Ahmadvand3, Davood Nourabadi4, Alireza Nakhaei1. 1. Department of Pharmacology, Zahedan University of Medical Sciences, Zahedan, Iran. 2. Department of Physiology, Zahedan University of Medical Sciences, Zahedan, Iran. 3. Department of Biochemistry, Faculty of Medicine, Lorestan University of Medical Sciences, Khorram Abad, Iran. 4. Department of Physiology, Iran University of Medical Sciences, Tehran, Iran.
Abstract
BACKGROUND: We investigated the effects of Withania somnifera root (WS) on insulin resistance, tumor necrosis factor α (TNF-α), and interleukin-6 (IL-6) in fructose-fed rats. METHODS: Forty-eight Wistar-Albino male rats were randomly divided into four groups (n=12); Group I as control, Group II as sham-treated with WS by 62.5mg/g per diet, Group III fructose-fed rats received 10%W/V fructose, and Group IV fructose- and WS-fed rats. After eight weeks blood samples were collected to measure glucose, insulin, IL-6, and TNF-α levels in sera. RESULTS: Blood glucose, insulin, homeostasis model assessment for insulin resistance (HOMA-R), IL-6, and TNF-α levels were all significantly greater in the fructose-fed rats than in the controls. Treatment with WS significantly (P < 0.05) inhibited the fructose-induced increases in glucose, insulin, HOMA-R, IL-6, and TNF-α. CONCLUSION: Our data suggest that WS normalizes hyperglycemia in fructose-fed rats by reducing inflammatory markers and improving insulin sensitivity.
BACKGROUND: We investigated the effects of Withania somnifera root (WS) on insulin resistance, tumornecrosis factor α (TNF-α), and interleukin-6 (IL-6) in fructose-fed rats. METHODS: Forty-eight Wistar-Albino male rats were randomly divided into four groups (n=12); Group I as control, Group II as sham-treated with WS by 62.5mg/g per diet, Group III fructose-fed rats received 10%W/V fructose, and Group IV fructose- and WS-fed rats. After eight weeks blood samples were collected to measure glucose, insulin, IL-6, and TNF-α levels in sera. RESULTS:Blood glucose, insulin, homeostasis model assessment for insulin resistance (HOMA-R), IL-6, and TNF-α levels were all significantly greater in the fructose-fed rats than in the controls. Treatment with WS significantly (P < 0.05) inhibited the fructose-induced increases in glucose, insulin, HOMA-R, IL-6, and TNF-α. CONCLUSION: Our data suggest that WS normalizes hyperglycemia in fructose-fed rats by reducing inflammatory markers and improving insulin sensitivity.