Kheirollah Yari1, Ziba Rahimi1, Mehrdad Payandeh1, Zohreh Rahimi2. 1. Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran. 2. Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran; Department of Clinical Biochemistry, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Abstract
BACKGROUND: Matrix metalloproteinases (MMPs) are upregulated in tumors. The MMP-7 A-181G polymorphism is associated with increased expression of the MMP-7 gene. Aim of the present study was to investigate the association between the MMP-7 A-181G polymorphism and susceptibility to breast cancer. PATIENTS AND METHODS: The MMP-7 A-181G variants were studied in a cohort of 251 subjects consisting of 100 breast cancer patients and 151 healthy controls; all were from Western Iran. The MMP-7 A-181G genotypes were identified using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. RESULTS: The frequencies of the MMP-7 AA, AG, and GG genotypes in healthy individuals were 34.4, 50.4, and 15.2%, respectively. In breast cancer patients, the frequencies of AA (34%), AG (52%), and GG (14%) genotypes (p = 0.95) were similar to those in the controls. There was a trend toward an increased frequency of the combined genotype of MMP-7 AG+GG in patients with lymph node metastasis (70.4%) compared to those without metastasis (66.7%). Also, in patients with invasive lobular carcinoma, the frequency of the MMP-7 AG+GG genotype tended to be higher (71.4%) compared to that in patients with invasive ductal carcinoma (66.2%) (p = 0.78). CONCLUSION: Our findings indicate that the MMP-7 A-181G polymorphism may not be correlated with susceptibility to breast cancer in our population.
BACKGROUND: Matrix metalloproteinases (MMPs) are upregulated in tumors. The MMP-7A-181G polymorphism is associated with increased expression of the MMP-7 gene. Aim of the present study was to investigate the association between the MMP-7A-181G polymorphism and susceptibility to breast cancer. PATIENTS AND METHODS: The MMP-7A-181G variants were studied in a cohort of 251 subjects consisting of 100 breast cancerpatients and 151 healthy controls; all were from Western Iran. The MMP-7A-181G genotypes were identified using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. RESULTS: The frequencies of the MMP-7 AA, AG, and GG genotypes in healthy individuals were 34.4, 50.4, and 15.2%, respectively. In breast cancerpatients, the frequencies of AA (34%), AG (52%), and GG (14%) genotypes (p = 0.95) were similar to those in the controls. There was a trend toward an increased frequency of the combined genotype of MMP-7 AG+GG in patients with lymph node metastasis (70.4%) compared to those without metastasis (66.7%). Also, in patients with invasive lobular carcinoma, the frequency of the MMP-7 AG+GG genotype tended to be higher (71.4%) compared to that in patients with invasive ductal carcinoma (66.2%) (p = 0.78). CONCLUSION: Our findings indicate that the MMP-7A-181G polymorphism may not be correlated with susceptibility to breast cancer in our population.
Entities:
Keywords:
Breast cancer; Gene polymorphism; MMP-7 A-181G; Western Iran
Authors: Ping Zhou; Liang-Feng Du; Guo-Qiang Lv; Xian-Ming Yu; Yuan-Long Gu; Jian-Ping Li; Chun Zhang Journal: Breast Cancer Res Treat Date: 2010-12-15 Impact factor: 4.872
Authors: Mohammad R Motie; Sima Besharat; Reza Torkjazi; Mahdieh Shojaa; Mahsa Besharat; Abbasali Keshtkar; Gholamreza Roshandel; Saba Besharat; Arab Ameri Fateme Journal: Breast Care (Basel) Date: 2011-12-15 Impact factor: 2.860