Leandro T Retamoso1, Mauro E P Silveira2, Frederico D Lima3, Guilherme L Busanello1, Guilherme Bresciani4, Leandro R Ribeiro5, Pietro M Chagas6, Cristina W Nogueira6, Ana Claudia M Braga7, Ana Flávia Furian7, Mauro S Oliveira7, Michele R Fighera8, Luiz Fernando F Royes9. 1. Laboratório de Bioquímica do Exercício, Departamento de Métodos e Técnicas Desportivas, Centro de Educação Física e Desportos, Universidade Federal de Santa Maria, RS, Brazil; Programa de Pós-Graduação em Educação Física, Centro de Educação Física e Desportos, Universidade Federal de Santa Maria, RS, Brazil. 2. Laboratório de Bioquímica do Exercício, Departamento de Métodos e Técnicas Desportivas, Centro de Educação Física e Desportos, Universidade Federal de Santa Maria, RS, Brazil; Programa de Pós-Graduacão em Ciências Biológicas: Bioquímica Toxicológica, Universidade Federal de Santa Maria, RS, Brazil. 3. Laboratório de Bioquímica do Exercício, Departamento de Métodos e Técnicas Desportivas, Centro de Educação Física e Desportos, Universidade Federal de Santa Maria, RS, Brazil. 4. Instituto de Actividad Física y Salud, Facultad de Ciencias de la Salud, Universidad Autónoma de Chile, Temuco, Chile. 5. Laboratório de Bioquímica do Exercício, Departamento de Métodos e Técnicas Desportivas, Centro de Educação Física e Desportos, Universidade Federal de Santa Maria, RS, Brazil; Programa de Pós-graduacão em Neurociências, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, SC, Brazil. 6. Programa de Pós-Graduacão em Ciências Biológicas: Bioquímica Toxicológica, Universidade Federal de Santa Maria, RS, Brazil. 7. Programa de Pós-graduacão em Farmacologia, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, RS, Brazil. 8. Departamento de Neuropsiquiatria, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, RS, Brazil. 9. Laboratório de Bioquímica do Exercício, Departamento de Métodos e Técnicas Desportivas, Centro de Educação Física e Desportos, Universidade Federal de Santa Maria, RS, Brazil; Programa de Pós-Graduacão em Ciências Biológicas: Bioquímica Toxicológica, Universidade Federal de Santa Maria, RS, Brazil. Electronic address: nandoroyes@yahoo.com.br.
Abstract
AIMS: It is well-known that unaccustomed exercise, especially eccentric exercise, is associated to delayed onset muscle soreness (DOMS). Whether DOMS is associated with reactive oxygen species (ROS) and the transient receptor potential vanilloid 1 (TRPV1) is still an open question. Thus, the aim of this study was to investigate the association between TRPV1 and xanthine oxidase-related ROS production in muscle and DOMS after a bout of eccentric exercise. MAIN METHODS: Male Wistar rats performed a downhill running exercise on a treadmill at a -16° tilt and a constant speed for 90min (5min/bout separated by 2min of rest). Mechanical allodynia and grip force tests were performed before and 1, 3, 6, 9, 12, 24, 48 and 72h after the downhill running. Biochemical assays probing oxidative stress, purine degradation, xanthine oxidase activity, Ca(2+) ATPase activity and TRPV1 protein content were performed in gastrocnemius muscle at 12, 24, and 48h after the downhill running. KEY FINDINGS: Our statistical analysis showed an increase in mechanical allodynia and a loss of strength after the downhill running. Similarly, an increase in carbonyl, xanthine oxidase activity, uric acid levels and TRPV1 immunoreactivity were found 12h post-exercise. On the other hand, Ca(2+) ATPase activity decreased in all analyzed times. SIGNIFICANCE: Our results suggest that a possible relationship between xanthine oxidase-related ROS and TRPV1 may exist during the events preceding eccentric exercise-related DOMS.
AIMS: It is well-known that unaccustomed exercise, especially eccentric exercise, is associated to delayed onset muscle soreness (DOMS). Whether DOMS is associated with reactive oxygen species (ROS) and the transient receptor potential vanilloid 1 (TRPV1) is still an open question. Thus, the aim of this study was to investigate the association between TRPV1 and xanthine oxidase-related ROS production in muscle and DOMS after a bout of eccentric exercise. MAIN METHODS: Male Wistar rats performed a downhill running exercise on a treadmill at a -16° tilt and a constant speed for 90min (5min/bout separated by 2min of rest). Mechanical allodynia and grip force tests were performed before and 1, 3, 6, 9, 12, 24, 48 and 72h after the downhill running. Biochemical assays probing oxidative stress, purine degradation, xanthine oxidase activity, Ca(2+) ATPase activity and TRPV1 protein content were performed in gastrocnemius muscle at 12, 24, and 48h after the downhill running. KEY FINDINGS: Our statistical analysis showed an increase in mechanical allodynia and a loss of strength after the downhill running. Similarly, an increase in carbonyl, xanthine oxidase activity, uric acid levels and TRPV1 immunoreactivity were found 12h post-exercise. On the other hand, Ca(2+) ATPase activity decreased in all analyzed times. SIGNIFICANCE: Our results suggest that a possible relationship between xanthine oxidase-related ROS and TRPV1 may exist during the events preceding eccentric exercise-related DOMS.
Authors: Helenita Antonia de Oliveira; Ednei Luiz Antonio; Gisela Arsa; Eduardo Tadeu Santana; Flávio André Silva; Daniel Arruda Júnior; Simone Dos Santos; Paulo de Tarso Camillo de Carvalho; Ernesto Cesar Pinto Leal-Junior; Amanda Araujo; Kátia De Angelis; Danilo Sales Bocalini; José Antonio Silva Junior; Paulo José Ferreira Tucci; Andrey Jorge Serra Journal: Oxid Med Cell Longev Date: 2018-02-13 Impact factor: 6.543