Shahzad Irfan1,2, Jens Ehmcke1,3, Muhammad Shahab2, Joachim Wistuba1, Stefan Schlatt1. 1. Institute of Reproductive and Regenerative Biology, Center of Reproductive Medicine and Andrology, University Clinics, Műnster, Germany. 2. Department of Animal Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan. 3. Central Animal Facility of the Faculty of Medicine, University of Münster, Münster, Germany.
Abstract
BACKGROUND: Hypothalamic kisspeptin-kisspeptin receptor signalling in primates ensures the successful progression into puberty during development and maintenance of reproductive capacity during adulthood. Human testis has been shown to express high-to-moderate levels of kisspeptin and kisspeptin receptor gene expression. In this study, we aimed at characterizing the localization of kisspeptin and kisspeptin receptor in adult primate testis tissue. METHODS: Immunocytochemistry was performed on paraffin-embedded testicular sections from adult rhesus monkeys and from common marmoset monkeys. RESULTS: Kisspeptin receptor was detected in Sertoli cells in the periphery of the seminiferous tubules in adult testes of both species. In contrast, kisspeptin was not localized in the seminiferous epithelium and was detected only in the interstitial compartment of the adult rhesus monkey testis. CONCLUSION: Kisspeptin receptor and kisspeptin are localized in the testis of Old World and New World primates.
BACKGROUND: Hypothalamic kisspeptin-kisspeptin receptor signalling in primates ensures the successful progression into puberty during development and maintenance of reproductive capacity during adulthood. Human testis has been shown to express high-to-moderate levels of kisspeptin and kisspeptin receptor gene expression. In this study, we aimed at characterizing the localization of kisspeptin and kisspeptin receptor in adult primate testis tissue. METHODS: Immunocytochemistry was performed on paraffin-embedded testicular sections from adult rhesus monkeys and from common marmoset monkeys. RESULTS: Kisspeptin receptor was detected in Sertoli cells in the periphery of the seminiferous tubules in adult testes of both species. In contrast, kisspeptin was not localized in the seminiferous epithelium and was detected only in the interstitial compartment of the adult rhesus monkey testis. CONCLUSION: Kisspeptin receptor and kisspeptin are localized in the testis of Old World and New World primates.
Authors: Eun Bee Lee; Iman Dilower; Courtney A Marsh; Michael W Wolfe; Saeed Masumi; Sameer Upadhyaya; Mohammad A Karim Rumi Journal: Cells Date: 2022-03-28 Impact factor: 6.600
Authors: Saeed Masumi; Eun Bee Lee; Iman Dilower; Sameer Upadhyaya; V Praveen Chakravarthi; Patrick E Fields; M A Karim Rumi Journal: Front Endocrinol (Lausanne) Date: 2022-08-22 Impact factor: 6.055