Roland Assi1, Trenton R Foster1, Hao He1,2, Katerina Stamati3, Hualong Bai1, Yuegao Huang4, Fahmeed Hyder4, Douglas Rothman4, Chang Shu2, Shervanthi Homer-Vanniasinkam3, Umber Cheema3, Alan Dardik1,5. 1. Vascular Biology & Therapeutics Program & the Department of Surgery, Yale University School of Medicine, New Haven, CT, USA. 2. Department of Vascular Surgery, The Second Xiangya Hospital of Central South University, Changsha, China. 3. UCL Institute of Orthopaedics & Musculoskeletal Sciences, UCL Division of Surgery & Interventional Sciences, University College London, London, UK. 4. Departments of Diagnostic Radiology & Biomedical Engineering, Yale University, New Haven, CT, USA. 5. Department of Surgery, VA Connecticut Healthcare Systems, West Haven, CT, USA.
Abstract
AIM: We hypothesized that delivery of mesenchymal stem cells (MSCs) in a biomimetic collagen scaffold improves wound healing in a diabetic mouse model. MATERIALS & METHODS: Rolled collagen scaffolds containing MSCs were implanted or applied topically to diabetic C57BL/6 mice with excisional wounds. RESULTS: Rolled scaffolds were hypoxic, inducing MSC synthesis and secretion of VEGF. Diabetic mice with wounds treated with rolled scaffolds containing MSCs showed increased healing compared with controls. Histologic examination showed increased cellular proliferation, increased VEGF expression and capillary density, and increased numbers of macrophages, fibroblasts and smooth muscle cells. Addition of laminin to the collagen scaffold enhanced these effects. CONCLUSION: Activated MSCs delivered in a biomimetic-collagen scaffold enhanced wound healing in a translationally relevant diabetic mouse model.
AIM: We hypothesized that delivery of mesenchymal stem cells (MSCs) in a biomimetic collagen scaffold improves wound healing in a diabetic mouse model. MATERIALS & METHODS: Rolled collagen scaffolds containing MSCs were implanted or applied topically to diabetic C57BL/6 mice with excisional wounds. RESULTS: Rolled scaffolds were hypoxic, inducing MSC synthesis and secretion of VEGF. Diabetic mice with wounds treated with rolled scaffolds containing MSCs showed increased healing compared with controls. Histologic examination showed increased cellular proliferation, increased VEGF expression and capillary density, and increased numbers of macrophages, fibroblasts and smooth muscle cells. Addition of laminin to the collagen scaffold enhanced these effects. CONCLUSION: Activated MSCs delivered in a biomimetic-collagen scaffold enhanced wound healing in a translationally relevant diabetic mouse model.
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