| Literature DB >> 2698650 |
K S Prickett1, D C Amberg, T P Hopp.
Abstract
We report a straightforward methodology for purification of recombinant proteins by incorporating a short hydrophilic peptide marker segment at their N-termini. A calcium-dependent antibody that reacts primarily with the first three amino acids of this peptide segment was used to affinity purify the fusion proteins in a single chromatographic step. The marker peptide could subsequently be removed by proteolysis with the enzyme enterokinase.Entities:
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Year: 1989 PMID： 2698650
Source DB: PubMed Journal: Biotechniques ISSN： 0736-6205 Impact factor: 1.993