| Literature DB >> 26982821 |
Yang-Nim Park1, Rebecca A Glover1, Karla J Daniels1, David R Soll1.
Abstract
The maltose binding protein (MBP) is a commonly used protein tag. Two monoclonal antibodies (mAbs) were generated against the MBP by immunizing mice with purified 6xHis-tagged MBP (6xHis-MBP). A nontoxic adjuvant cocktail of poly(I:C) and anti-CD40 mAb was used. The two mAbs, 3D7 and 2A1, are demonstrated to be effective in immunoprecipitation, immunoblotting, western blot hybridization, and the ELISA assay. These two mAbs are available individually or in combination at cost through the Developmental Studies Hybridoma Bank, a nonprofit National Resource created by the National Institutes of Health.Entities:
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Year: 2016 PMID： 26982821 PMCID： PMC4845686 DOI： 10.1089/mab.2015.0072
Source DB: PubMed Journal: Monoclon Antib Immunodiagn Immunother ISSN： 2167-9436