Alper Tekeli1, Duygu Nilufer Ocal2, Busra Betul Ozmen1, Zeynep Ceren Karahan1,3, Istar Dolapci1. 1. 1 Department of Medical Microbiology, Ankara University School of Medicine , Ankara, Turkey . 2. 2 Department of Microbiology, Ankara Diskapi Yildirim Beyazit Training and Research Hospital , Ankara, Turkey . 3. 3 Central Bacteriology Laboratory, İbn-i Sina Hospital , Ankara, Turkey .
Abstract
AIMS: Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most important pathogens in the hospital environment. Monitoring of this pathogen by molecular characterization and phenotypic methods is important for the development of suitable infection control measures and proper therapy design. In this study, our aim was to investigate the molecular epidemiological characteristics of MRSA bloodstream isolates obtained from patients hospitalized at Ankara University Ibn-i Sina Hospital in a 10-year period (2002-2012) and monitor the possible changes. A total of 134 isolates were characterized according to their antimicrobial susceptibility profiles, biofilm formation capabilities, accessory gene regulator (agr) locus types, presence of genes encoding Panton-Valentine leukocidin (PVL), staphylococcal enterotoxins A-J (SEs A-J), toxic shock syndrome toxin, sasX, and genes associated with biofilm formation (icaD, icaA, IS256) by polymerase chain reaction. The staphylococcal cassette chromosome mec (SCCmec) types of isolates were also defined and their clonal relationships were investigated by pulsed-field gel electrophoresis (PFGE) analysis and multilocus sequence typing was performed for representative isolates obtained by PFGE. RESULTS: The majority of the isolates were resistant to rifampin (100%), ciprofloxacin (97%), tetracycline (97.7%), and gentamicin (94.7%); 100% carried type-III SCCmec and 89.5% were agr type-1. All the isolates were negative for PVL, and sasX genes while all of them carried the icaD, icaA, and IS256 genes. The most common SE was enterotoxin A (97%). Four major PFGE patterns with the dominance of one pattern and seven unique patterns were obtained. All the representative PFGE isolates (n = 11) belonged to sequence type 239. CONCLUSION: We have documented the characteristics of the dominant MRSA clone in our hospital, which was a PVL (-), sasX (-) ST239 clone carrying sea (+) with type-III SCCmec, and type-1 agr locus.
AIMS: Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most important pathogens in the hospital environment. Monitoring of this pathogen by molecular characterization and phenotypic methods is important for the development of suitable infection control measures and proper therapy design. In this study, our aim was to investigate the molecular epidemiological characteristics of MRSA bloodstream isolates obtained from patients hospitalized at Ankara University Ibn-i Sina Hospital in a 10-year period (2002-2012) and monitor the possible changes. A total of 134 isolates were characterized according to their antimicrobial susceptibility profiles, biofilm formation capabilities, accessory gene regulator (agr) locus types, presence of genes encoding Panton-Valentine leukocidin (PVL), staphylococcal enterotoxins A-J (SEs A-J), toxic shock syndrome toxin, sasX, and genes associated with biofilm formation (icaD, icaA, IS256) by polymerase chain reaction. The staphylococcal cassette chromosome mec (SCCmec) types of isolates were also defined and their clonal relationships were investigated by pulsed-field gel electrophoresis (PFGE) analysis and multilocus sequence typing was performed for representative isolates obtained by PFGE. RESULTS: The majority of the isolates were resistant to rifampin (100%), ciprofloxacin (97%), tetracycline (97.7%), and gentamicin (94.7%); 100% carried type-III SCCmec and 89.5% were agr type-1. All the isolates were negative for PVL, and sasX genes while all of them carried the icaD, icaA, and IS256 genes. The most common SE was enterotoxin A (97%). Four major PFGE patterns with the dominance of one pattern and seven unique patterns were obtained. All the representative PFGE isolates (n = 11) belonged to sequence type 239. CONCLUSION: We have documented the characteristics of the dominant MRSA clone in our hospital, which was a PVL (-), sasX (-) ST239 clone carrying sea (+) with type-III SCCmec, and type-1 agr locus.
Authors: Stefan Monecke; Peter Slickers; Darius Gawlik; Elke Müller; Annett Reissig; Antje Ruppelt-Lorz; Patrick E Akpaka; Dirk Bandt; Michele Bes; Samar S Boswihi; David C Coleman; Geoffrey W Coombs; Olivia S Dorneanu; Vladimir V Gostev; Margaret Ip; Bushra Jamil; Lutz Jatzwauk; Marco Narvaez; Rashida Roberts; Abiola Senok; Anna C Shore; Sergey V Sidorenko; Leila Skakni; Ali M Somily; Muhammad Ali Syed; Alexander Thürmer; Edet E Udo; Teodora Vremerǎ; Jeannete Zurita; Ralf Ehricht Journal: Front Microbiol Date: 2018-07-06 Impact factor: 5.640
Authors: Marwa I Abd El-Hamid; Alaa H Sewid; Mohamed Samir; Wael A H Hegazy; Mosa M Bahnass; Rasha A Mosbah; Doaa M Ghaith; Eman Khalifa; Hazem Ramadan; Walaa A Alshareef; Hanan M Alshareef; Mohammed M Ghoneim; Mohammad M Al-Sanea; Mahmoud M Bendary Journal: Front Cell Infect Microbiol Date: 2022-03-25 Impact factor: 5.293