Tao Chen1, Zhenyan Li2, Li Yu3, Haicheng Li1, Jinfei Lin1, Huixin Guo1, Wei Wang4, Liang Chen1, Xianen Zhang5, Yunxia Wang6, Yuhui Chen1, Qinghua Liao5, Yaoju Tan7, Yang Shu1, Wenyan Huang6, Changhui Cai8, Zhongjing Zhou9, Meiling Yu9, Guozhou Li10, Lin Zhou11, Qiu Zhong12, Lijun Bi13, Meigui Zhao14, Lina Guo15, Jie Zhou16. 1. Center for Tuberculosis Control of Guangdong Province, Guangzhou 510630, China. 2. Department of Microbiology and Immunology, Jinan University, Guangzhou 510623, China. 3. College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China. 4. Foshan Fourth People's Hospital, Foshan 528000, China. 5. Institute of Biophysics, Chinese Academy of Science, Beijing 100101, China. 6. Bao'an Chronic Diseases Prevention and Cure Hospital, Shenzhen 518102, China. 7. Guangzhou Chest Hospital, Guangzhou 510095, China. 8. The Second People's Hospital of Zhongshan City, Zhongshan 528447, China. 9. Chinese Medicine Hospital of Huangpu District, Guangzhou 510700, China. 10. Chronic Disease Control and Prevention Station of Dongguan, Guangdong 523008, China. 11. Center for Tuberculosis Control of Guangdong Province, Guangzhou 510630, China. Electronic address: zhoulinpaper@163.com. 12. Center for Tuberculosis Control of Guangdong Province, Guangzhou 510630, China. Electronic address: zhongqiu@vip.163.com. 13. Institute of Biophysics, Chinese Academy of Science, Beijing 100101, China. Electronic address: blj@sun5.ibp.ac.cn. 14. Bao'an Chronic Diseases Prevention and Cure Hospital, Shenzhen 518102, China. Electronic address: bazmg@126.com. 15. GuangDong Provincial Hospital of Chinese Medicine, Guangdong 510120, China. Electronic address: gln408@126.com. 16. Foshan Fourth People's Hospital, Foshan 528000, China. Electronic address: zjet65@163.com.
Abstract
OBJECT: Tuberculosis (TB) continues to be one of the most serious infectious diseases in the world, however, no effective biomarkers can be used for rapid screening of latent tuberculosis infection (LTBI) and active TB. In this study, serum cytokines were screened and tested as potential biomarker for TB diagnosis. METHOD: Cytokine array was used to track the cytokine profile and its dynamic change after TB infection. The different expressions of cytokines were confirmed by ELISA assay. ROC curve analyses were used to evaluate the efficacy of a cytokine or cytokine combination for diagnosis. RESULTS: Eotaxin-2, ICAM-1, MCSF, IL-12p70, and IL-11 were significantly higher in the LTBI individuals. I-309, MIG, Eotaxin-2, IL-8, ICAM-1, IL-6sR, and Eotaxin were significantly higher in active TB patients. ROC curve analyses gave AUCs of 0.843, 0.898, and 0.888 for I-309, MIG, and IL-8, respectively, and 0.894 for the combination panel in active TB diagnosis. IFN-γ/IL-4 and IL-2/TNF-α ratios exhibit dynamic changes in the healthy control and LTBI to different stages of active TB. CONCLUSIONS: Serum cytokines, including I-309 and MIG, IL-8, Extoxin-2, ICAM-1 and combinations of cytokines, including IFN-γ/IL-4 and IL-2/TNF-α, can be used as serum biomarkers for LTBI and active TB screening, thus indicating prospective clinical applications.
OBJECT: Tuberculosis (TB) continues to be one of the most serious infectious diseases in the world, however, no effective biomarkers can be used for rapid screening of latent tuberculosis infection (LTBI) and active TB. In this study, serum cytokines were screened and tested as potential biomarker for TB diagnosis. METHOD: Cytokine array was used to track the cytokine profile and its dynamic change after TB infection. The different expressions of cytokines were confirmed by ELISA assay. ROC curve analyses were used to evaluate the efficacy of a cytokine or cytokine combination for diagnosis. RESULTS:Eotaxin-2, ICAM-1, MCSF, IL-12p70, and IL-11 were significantly higher in the LTBI individuals. I-309, MIG, Eotaxin-2, IL-8, ICAM-1, IL-6sR, and Eotaxin were significantly higher in active TB patients. ROC curve analyses gave AUCs of 0.843, 0.898, and 0.888 for I-309, MIG, and IL-8, respectively, and 0.894 for the combination panel in active TB diagnosis. IFN-γ/IL-4 and IL-2/TNF-α ratios exhibit dynamic changes in the healthy control and LTBI to different stages of active TB. CONCLUSIONS: Serum cytokines, including I-309 and MIG, IL-8, Extoxin-2, ICAM-1 and combinations of cytokines, including IFN-γ/IL-4 and IL-2/TNF-α, can be used as serum biomarkers for LTBI and active TB screening, thus indicating prospective clinical applications.
Authors: Ruschca Jacobs; Elizna Maasdorp; Stephanus Malherbe; Andre G Loxton; Kim Stanley; Gian van der Spuy; Gerhard Walzl; Novel N Chegou Journal: PLoS One Date: 2016-08-03 Impact factor: 3.240
Authors: Ruschca Jacobs; Stephanus Malherbe; Andre G Loxton; Kim Stanley; Gian van der Spuy; Gerhard Walzl; Novel N Chegou Journal: Oncotarget Date: 2016-09-06
Authors: Alexander V Panteleev; Irina Yu Nikitina; Irina A Burmistrova; George A Kosmiadi; Tatyana V Radaeva; Rasul B Amansahedov; Pavel V Sadikov; Yana V Serdyuk; Elena E Larionova; Tatef R Bagdasarian; Larisa N Chernousova; Vitaly V Ganusov; Irina V Lyadova Journal: Front Immunol Date: 2017-08-21 Impact factor: 7.561
Authors: Sasha E Larsen; Susan L Baldwin; Mark T Orr; Valerie A Reese; Tiffany Pecor; Brian Granger; Natasha Dubois Cauwelaert; Brendan K Podell; Rhea N Coler Journal: Vaccines (Basel) Date: 2018-05-24