Jhoti Somanah1, Srishti Ramsaha2, Shalini Verma3, Ashok Kumar4, Poornima Sharma5, Ranjan Kumar Singh5, Okezie I Aruoma6, Emmanuel Bourdon7, Theeshan Bahorun8. 1. ANDI Centre of Excellence for Biomedical and Biomaterials Research, MSIRI Building, Faculty of Science, University of Mauritius, Réduit, Mauritius. 2. Department of Health Sciences and ANDI Centre of Excellence for Biomedical and Biomaterials Research, Faculty of Science, University of Mauritius, Réduit, Mauritius. 3. Institute of Biosciences and Biotechnology, Chhatrapati Shahuji Maharaj University, Kanpur, Uttar Pradesh, India. 4. Deen Dayal Upadhyay Gorakhpur University, Gorakhpur, Uttar Pradesh, India. 5. Department of Physics, Banaras Hindu University, Varanasi, Uttar Pradesh, India. 6. School of Pharmacy and Biomedical Sciences, University of Long Beach, Long Beach, California, USA. 7. UMR DÉTROIT-INSERM U1188, Diabète Athérothrombose Thérapies Réunion Océan Indien, Université de La Réunion, La Réunion, France. 8. ANDI Centre of Excellence for Biomedical and Biomaterials Research, MSIRI Building, Faculty of Science, University of Mauritius, Réduit, Mauritius. Electronic address: tbahorun@uom.ac.mu.
Abstract
AIM AND MAIN METHOD: The medicinal properties of fermented papaya preparation (FPP) derived from Carica papaya fruit was investigated in order to determine its ability to modulate the progression of N-methyl-N-nitrosourea induced hepatocellular carcinoma in Balb/c mice. KEY FINDINGS: As well as reducing the physical symptoms associated with N-methyl-N-nitrosourea (MNU)-induced hepatocellular carcinoma, supplementation of Balb/c mice with 500mg FPP/kg BW for 92days normalized the blood cell count, led to an increased activity of several key antioxidant enzymes (SOD: +20%, CAT: +81%, GPx: +66.1%, GR: +54.4%; P<0.001 vs. MNU control), increased the ferrous reducing antioxidant potential (+36.7%, P<0.001 vs. MNU control) and reduced the extent of lipid peroxidation in the liver by 44.3% (P<0.001 vs. MNU control). SIGNIFICANCE: Results demonstrated the ability of FPP to preserve the integrity of liver against oxidative damage and protect hepatocytes against irreversible DNA structural modifications induced by MNU, highlighting its potential role as an immune-defense modulator during hepatocarcinoma.
AIM AND MAIN METHOD: The medicinal properties of fermented papaya preparation (FPP) derived from Carica papaya fruit was investigated in order to determine its ability to modulate the progression of N-methyl-N-nitrosourea induced hepatocellular carcinoma in Balb/c mice. KEY FINDINGS: As well as reducing the physical symptoms associated with N-methyl-N-nitrosourea (MNU)-induced hepatocellular carcinoma, supplementation of Balb/c mice with 500mg FPP/kg BW for 92days normalized the blood cell count, led to an increased activity of several key antioxidant enzymes (SOD: +20%, CAT: +81%, GPx: +66.1%, GR: +54.4%; P<0.001 vs. MNU control), increased the ferrous reducing antioxidant potential (+36.7%, P<0.001 vs. MNU control) and reduced the extent of lipid peroxidation in the liver by 44.3% (P<0.001 vs. MNU control). SIGNIFICANCE: Results demonstrated the ability of FPP to preserve the integrity of liver against oxidative damage and protect hepatocytes against irreversible DNA structural modifications induced by MNU, highlighting its potential role as an immune-defense modulator during hepatocarcinoma.
Authors: Yi Tian; Manuel A Riquelme; Chao Tu; Yumeng Quan; Xiaowen Liu; Lu-Zhe Sun; Jean X Jiang Journal: Cancers (Basel) Date: 2021-12-17 Impact factor: 6.639