Previous tonsillectomy modifies odds of tonsil and base of tongue cancer.

BACKGROUND: Tonsillectomy is a commonly performed surgical procedure that involves removal of the palatine tonsils. The purpose of this study is to examine the association between previous tonsillectomy and odds of oropharyngeal squamous cell carcinoma (OPSCC) in a large population-based case-control study. We hypothesise that previous tonsillectomy is associated with a decreased odds of tonsil cancer with no impact on the odds of developing base of tongue (BOT) cancer.
METHODS: This was a population-based, frequency-matched case-control study with multinomial logistic regression, including 1378 controls, 108 BOT cancer cases, and 198 tonsil cancer cases. Demographic and risk factor data were collected using a structured questionnaire during an in-home visit conducted by trained nurse-interviewers. The human papillomavirus (HPV) tumour status was determined through Luminex-based multiplex PCR and p16 status by immunohistochemistry.
RESULTS: Previous tonsillectomy was associated with a nearly two-fold increased odds of BOT cancer (OR=1.95, 95% CI 1.25-3.06, P=0.003) and a large decrease in the odds of tonsil cancer (OR=0.22, 95% CI 0.13-0.36, P<0.001). When HPV status was considered, tonsillectomy was associated with a decreased odds of HPV-positive tonsil cancer (OR=0.17, 95% CI 0.08-0.34, P<0.001) and an increased risk of HPV-positive BOT cancer (OR=2.46, 95% CI 1.22-4.95, P=0.012). When p16 status was considered, tonsillectomy was associated with an increased odds of p16-positive BOT cancer (OR=2.24, 95% CI 1.16-4.35, P=0.017) and a decreased odds of p16-positive tonsil cancer (OR=0.14, 95% CI 0.07-0.31, P<0.001).
CONCLUSIONS: Previous tonsillectomy modifies the odds of both tonsil and BOT cancer, with decreased odds of tonsil cancer and increased odds of BOT cancer. A history of previous tonsillectomy may play a role in OPSCC risk stratification when considered along with other covariates such as sexual history, smoking status, and age.

Oropharyngeal squamous cell carcinoma (OPSCC) is an increasingly common cancer of the upper aerodigestive tract, and includes cancers arising from the tonsils, base of tongue (BOT), soft palate, and lateral and posterior pharyngeal walls. Although traditionally associated with heavy tobacco and alcohol consumption, it is estimated that ∼60–70% of incident OPSCC cases are attributable to human papillomavirus (HPV) (D'Souza ; Ang ; Chaturvedi ). A rise in HPV-positive OPSCC has occurred since the 1980s in the United States that has been attributed to changing sexual practices beginning in the 1960s (Ernster ; Chaturvedi ). The HPV epidemic has changed the demographic characteristics of OPSCC patients considerably. Patients with HPV-positive OPSCC are generally younger than those with HPV-negative OPSCC and are often never smokers. An association with increased number of sexual partners and earlier sexual debut has also been demonstrated (D'Souza ; Heck ). The HPV-positive OPSCC is an increasingly significant public health problem; it is projected that by 2020 HPV-positive OPSCC will overtake cervical cancer as the most common HPV-related malignancy in the United States (Chaturvedi ).

Tonsillectomy is a commonly performed surgical procedure that involves removal of the palatine tonsils, most often in children for hypertrophic tonsils, chronic infection, or obstructive sleep apnoea (Derkay, 1993). The rates of tonsillectomy in the United States have changed significantly over the past several decades. Rosenfeld and Green (1990) and others (Derkay, 1993; Erickson ) demonstrated a sharp decline in tonsillectomy rates beginning in the late 1970s. It is well established that an increase in HPV-related OPSCC began to increase in the 1990s (Chaturvedi , 2011, 2013). The association between tonsillectomy and risk of OPSCC has not been extensively investigated. A recent study of the Danish Cancer Registry suggests a reduction in risk of tonsil cancer in patients with a previous history of tonsillectomy (Fakhry ). The purpose of this study is to examine the association between previous tonsillectomy and risk of OPSCC using a large North Carolina population-based head and neck cancer case–control study. We hypothesise that previous tonsillectomy is associated with a decreased risk of tonsil cancer with no impact on the risk of BOT cancer.

Materials and methods

Study population

The Carolina Head and Neck Cancer Study (CHANCE) is a population-based case–control study of squamous cell carcinoma of the head and neck (Divaris ). Case ascertainment utilised rapid ascertainment of newly diagnosed cancer cases through the North Carolina Central Cancer Registry. Cases were diagnosed with primary squamous cell carcinoma of the oral cavity, pharynx, and larynx between 1 January 2002 and 28 February 2006, were aged 20 to 80 years at diagnosis, and resided in a 46-county region in central North Carolina. Benign tumours, carcinomas in situ, papillary carcinomas, and adenocarcinomas were excluded. Controls were identified through the North Carolina Department of Motor Vehicle records and were frequency matched with cases on age, race, and sex.

The Carolina Head and Neck Cancer Study enrolled 1368 total cases; however, as we are only interested in OPSCC, we restricted the tumour site to only the oropharynx (N=372). Race other than European American and African American were excluded from the analysis because of sparse numbers (N=11). As we are interested specifically in the OPSCC subsites, cases were classified into two categories: (1) BOT: C01.9 and C02.4 and (2) tonsil: C09.0, C09.1, C09.8, and C09.9. We excluded OPSCC of the vallecular (C10.0; N=10), soft palate (C05.1, C05.2; N=23), and unspecified subsites (C10.8 and C10.9; N=23) because of small sample size. The HPV typing and p16 immunohistochemistry was performed on all cases of oropharyngeal tumours for which tumour blocks were available (N=213). All European-American and African-American controls were included in the analysis (N=1378). This study was approved by the institutional review board at the University of North Carolina at Chapel Hill.

Questionnaire and clinical assessment

Demographic, lifestyle, oral health, diet, and other risk factor information were collected using a structured questionnaire during an in-home visit conducted by trained nurse-interviewers. The median time between diagnosis and in-home interview was 4 months (interquartile range: 3–6 months). Two questions addressed a history of previous tonsillectomy, including, ‘Were your tonsils removed?' and ‘How old were you when tonsils removed?' For subjects who were unable to recall the exact age of tonsillectomy, the subject was able to answer child, teenager, or adult. Age at tonsillectomy was defined as before 13 years of age (child) and after 13 years of age (teenager and adult). The CHANCE questionnaire focussed on lifestyle and medical and family history risk factors. Interviewers did not collect information on tonsillectomy performed as part of the diagnostic workup or OPSCC therapy. Confounders to be adjusted for in statistical models were selected a priori based on their potential association with risk of OPSCC and tonsillectomy. Confounders obtained from the questionnaire included: age race, sex, smoking (<10 pack-years and ⩾10 pack-years), insurance status (private, Medicare/Medicaid, none, and other), education (some high school, completed high school, some college and above), alcohol (1 drink or less per week/>1 drink a week), and number of sexual partners (0–1, 2–5, 6–14, and 14+).

p16 immunohistochemistry and HPV typing

Immunohistochemistry (IHC) for p16 was performed according to the protocol provided with the CINtec Histology p16INK4a Kit (Ref 9511, mtm laboratories, Heidelberg, Germany) for the qualitative detection of the p16 antigen on slides prepared from formalin-fixed, paraffin-embedded biopsies. The p16 IHC scoring method used in this study was developed based on the methodology by Koo ), where expression was scored based on the percentage (0%=0, 1–10%=1, 11–50%=2, 51–80%=3, and 81–100%=4) and intensity (none=0, weak=1, moderate=2, and strong=3) of nuclear or cytoplasmic staining, and a combined score of ⩾4 was considered positive for p16 overexpression. This algorithm has been previously published in a study from the International Agency for Research on Cancer (IARC) (Anantharaman ), and has been compared with other p16 scoring methods with similar results (Weinberger ; Smeets ; Singhi and Westra, 2010). The HPV typing was determined through DNA extraction and genotyping by Luminex-based multiplex PCR (TS-E7-MPG, IARC, Lyon, France) (Gheit ; Schmitt , 2010). Tumours were classified as HPV-positive if they were positive for HPV16 or HPV18.

Statistical analysis

Differences in descriptive statistics by OPSCC subsite were estimated using the χ2 test and Fisher's exact test when cells were sparse. Unconditional logistic regression was used to calculate odds ratio (OR) adjusted with the confounders and matching factors: age, race, and gender. Subsite-specific adjusted ORs were calculated with unconditional multinomial logistic regression. To further explore the role of HPV, conditional logistic regression was used to calculate HPV/p16 status and subsite stratified ORs based on the matching factors, including age (defined in 7 categories), race (White/Black), and sex. All statistical analyses were implemented using SAS 9.3 (SAS Institute, Cary, NC, USA).

Results

The study population comprised 1378 controls and 361 OPSCC cases, including 108 BOT and 198 tonsil cancer cases. Table 1 shows the demographic, risk factor, and tumour characteristics of the controls and cases by subsite. Cases and controls differed with respect to sex, age, tobacco pack-years, education level, insurance status, age at tonsillectomy, and rates of previous tonsillectomy (40% vs 26.3% in controls and cases, respectively, P<0.001). Among study subjects with a history of tonsillectomy, 2 (2.5%) cases and 6 (1.1%) controls underwent the procedure within 10 years of enrolment in the study. No significant differences were noted between BOT and tonsil cancer cases with respect to age, sex, race, education and insurance status, p16 tumour status, and HPV tumour status. A history of previous tonsillectomy differed significant between tonsil (11.3%) and BOT (53.3%) cancer cases (P<0.0001). The BOT cancer cases with a history of tonsillectomy were significantly more likely to have undergone the procedure in childhood (92.9% among BOT cases and 68.2% among tonsil cancer cases, P<0.001).

Table 1

Descriptive statistics and comparisons between controls and all oropharynx cases and comparisons by subsite

	Control (N=1378)	All oropharynx (N=361)		Base of tongue (N=108)	Tonsil (N=198)
	N (%)	N (%)	P-value	N (%)	N (%)	P-value
Tonsillectomy
No	815 (60%)	260 (73.7%)	<0.001	49 (46.7%)	173 (88.7%)	<0.001
Yes	544 (40%)	93 (26.3%)		56 (53.3%)	22 (11.3%)
Missing	19	8		3	3
Race
White	1114 (80.8%)	277 (76.7%)	0.082	88 (81.5%)	153 (77.3%)	0.390
Black	264 (19.2%)	84 (23.3%)		20 (18.5%)	45 (22.7%)
Education
Some High School	217 (15.7%)	98 (27.1%)	<0.001	22 (20.4%)	55 (27.8%)	0.192
Completed High School	332 (24.1%)	97 (26.9%)		28 (25.9%)	57 (28.8%)
Some College and above	829 (60.2%)	166 (46%)		58 (53.7%)	86 (43.4%)
Tobacco pack-years
⩽10 Pack-years	765 (55.8%)	112 (31.4%)	<0.001	36 (33.3%)	63 (32%)	0.809
>10 Pack-years	606 (44.2%)	245 (68.6%)		72 (66.7%)	134 (68%)
Missing	7	4			1
Sex
Male	960 (69.7%)	299 (82.8%)	<0.001	95 (88%)	165 (83.3%)	0.279
Female	418 (30.3%)	62 (17.2%)		13 (12%)	33 (16.7%)
Insurance
Private	556 (40.4%)	181 (50.3%)	<0.001	59 (54.6%)	104 (52.5%)	0.980
Medicaid/Medicare	437 (31.7%)	93 (25.8%)		24 (22.2%)	44 (22.2%)
None	76 (5.5%)	40 (11.1%)		11 (10.2%)	22 (11.1%)
Other	308 (22.4%)	46 (12.8%)		14 (13%)	28 (14.1%)
Missing	1	1
Tumour p16 status
Positive	–	84 (34.0%)	–	46 (65.7%)	99 (69.2%)	0.6051
Negative	–	163 (66.0%)		24 (34.3%)	44 (30.8%)
Missing	1378	125		38	55
Tumour human papillomavirus (HPV) status
Positive	–	168 (67.7%)	–	43 (60.6%)	104 (72.7%)	0.0708
Negative	–	80 (32.3%)		28 (39.4%)	39 (27.3%)
Missing	1378	124		37	55
Age
<50 Years	156 (11.3%)	86 (23.8%)	<0.001	18 (16.7%)	52 (26.3%)	0.397
50–54 Years	161 (11.7%)	84 (23.3%)		29 (26.9%)	50 (25.3%)
55–59 Years	207 (15%)	69 (19.1%)		21 (19.4%)	42 (21.2%)
60–64 Years	205 (14.9%)	53 (14.7%)		17 (15.7%)	22 (11.1%)
65–69 Years	247 (17.9%)	35 (9.7%)		10 (9.3%)	18 (9.1%)
70–74 Years	231 (16.8%)	25 (6.9%)		10 (9.3%)	10 (5.1%)
>75 Years	171 (12.4%)	9 (2.5%)		3 (2.8%)	4 (2%)
Age at tonsillectomy
Child	417 (77.1%)	76 (82.6%)	<0.001	52 (92.9%)	15 (68.2%)	<0.001
Teenager	37 (6.8%)	5 (5.4%)		1 (1.8%)	3 (13.6%)
Adult	87 (16.1%)	11 (12.0%)		3 (5.4%)	4 (18.2%)
No tonsillectomy	815	260		49	173
Missing	22	9		3	3

Table 2 presents the results of the logistic regression model for selected factors and the odds of OPSCC by subsite. A greater number of sexual partners and >10 pack-year tobacco history were associated with an increased odds of all OPSCC, tonsil, and BOT cancers compared with controls. When all OPSCC cases were evaluated, there was a significantly decreased odds of cancer associated with previous tonsillectomy (OR=0.63, 95% confidence interval (CI) 0.47–0.85, P=0.003). When OPSCC subsite was considered, previous tonsillectomy was associated with a nearly two-fold increased odds of BOT cancer (OR=1.95, 95% CI 1.25–3.06, P=0.003) and a large decrease in the odds of tonsil cancer (OR=0.22, 95% CI 0.13–0.36, P<0.001). We found tonsillectomy not associated with sites of head and neck cancer not commonly associated with HPV (Supplementary Table S1). We then considered whether age at tonsillectomy affected the risk of OPSCC by subsite (Table 3). Childhood tonsillectomy (age <13 years) was strongly associated with increased odds of BOT cancer (adjusted OR=2.46, 95% CI 1.55–3.92, P<0.001), and no association was noted with tonsillectomy after age 13 years (OR=0.33, 95% CI 0.08–1.42, P=0.138). Tonsillectomy before age 13 years (OR=0.19, 95% CI 0.10–0.33, P<0.001) and after age 13 years (OR=0.32, 95% CI 0.14–01.00, P=0.017) was associated with a significantly decreased odds of tonsil cancer.

Table 2

Adjusted odds ratio for all oropharyngeal cancer cases and by subsite compared with controlsa

	Oropharyngeal cancer		Base of tongue cancer		Tonsil cancer
	OR (95% CI)	P-value	OR (95% CI)	P-value	OR (95% CI)	P-value
Tonsillectomy
No	Ref	–	Ref	–	Ref	–
Yes	0.63 (0.47–0.85)	0.003	1.95 (1.25–3.06)	0.003	0.22 (0.13–0.36)	<0.001
Number of sexual partners
0–1	Ref	–	Ref	–	Ref	–
2–5	2.44 (1.40–4.27)	0.002	2.1 (0.84–5.21)	0.110	3.44 (1.5–7.91)	0.004
6–14	3.81 (2.19–6.60)	<0.001	3.42 (1.42–8.27)	0.006	5.69 (2.49–13)	<0.001
14+	4.65 (2.65–8.18)	<0.001	3.75 (1.52–9.21)	0.004	7.73 (3.33–17.96)	<0.001
Education
Some High School	Ref	–	Ref	–	Ref	–
Completed High School	0.61 (0.41–0.91)	–0.015	0.67 (0.34–1.32)	–0.248	0.59 (0.36–0.97)	–0.037
Some College and above	0.45 (0.31–0.66)	<0.001	0.54 (0.29–1.03)	0.061	0.4 (0.25–0.64)	<0.001
Pack-years
<10 Pack-years	Ref	–	Ref	–	Ref	–
⩾10 Pack-years	2.2 (1.62–2.97)	<0.001	1.91 (1.18–3.09)	0.009	2.23 (1.5–3.32)	<0.001
Insurance
Private	Ref	–	Ref	–	Ref	–
Medicaid/Medicare	0.84 (0.57–1.25)	0.393	0.59 (0.3–1.13)	0.113	0.78 (0.46–1.31)	0.341
None	0.92 (0.55–1.53)	0.743	0.86 (0.37–1.98)	0.717	0.93 (0.51–1.72)	0.823
Other	0.7 (0.45–1.09)	0.112	0.6 (0.3–1.19)	0.1434	0.81 (0.47–1.41)	0.464
Alcohol
⩽1 Drink per week	Ref	–	Ref	–	Ref	–
>1 Drink per week	1.72 (1.16–2.56)	0.007	1.23 (0.67–2.27)	0.503	1.67 (0.98–2.84)	0.058

Abbreviations: CI=confidence interval; OR=odds ratio; Ref=reference value.

Odds ratios are all mutually adjusted for in addition to matching factors (age, race, and gender).

Table 3

Adjusted odds ratios for age at tonsillectomy by oropharyngeal subsite compared with controlsa

	Base of tongue		Tonsil
	OR (95% CI)	P-value	OR (95% CI)	P-value
Age at tonsillectomy
No tonsillectomy	Ref	–	Ref	–
<13 years	2.46 (1.55–3.92)	<0.001	0.19 (0.1–0.34)	<0.001
⩾13 years	0.33 (0.08–1.42)	0.138	0.32 (0.14–0.72)	0.006
Number of sexual partners
0–1	Ref	–	Ref	–
2–5	2.22 (0.89–5.54)	0.087	3.46 (1.5–7.95)	0.004
6–14	3.5 (1.45–8.45)	0.005	5.71 (2.5–13.05)	<0.001
14+	3.96 (1.61–9.73)	0.003	7.8 (3.36–18.15)	<0.001
Education
Some High School	Ref	–	Ref	–
Completed High School	0.66 (0.34–1.31)	0.234	0.59 (0.36–0.96)	0.034
Some College and above	0.5 (0.26–0.94)	0.031	0.4 (0.25–0.64)	<0.001
Pack-years
<10 Pack-years	Ref	–	Ref	–
⩾10 Pack-years	1.88 (1.16–3.04)	0.011	1.66 (0.98–2.83)	0.061
Insurance
Private	Ref	–	Ref	–
Medicaid/Medicare	0.59 (0.31–1.15)	0.120	0.78 (0.47–1.32)	0.360
None	0.81 (0.35–1.87)	0.624	0.92 (0.5–1.7)	0.797
Other	0.59 (0.3–1.18)	0.138	0.81 (0.47–1.41)	0.457
Alcohol
⩽1 drink per week	Ref	–	Ref	–
>1 drink per week	1.30 (0.7–2.39)	0.402	2.24 (1.51–3.32)	<0.001

Abbreviations: CI=confidence interval; OR=odds ratio; Ref=reference value.

Odds ratios are all mutually adjusted for in addition to matching factors (age, race, and gender).

In an exploratory analysis including cases with available tumour tissue, we then examined the odds of tonsil and BOT cancer by HPV and by p16 status (Table 4). Tonsillectomy was associated with a decreased odds of HPV-positive tonsil cancer (OR=0.17, 95% CI 0.08–0.34, P<0.001) and a decreased but imprecise odds of HPV-negative tonsil cancer (OR=0.66, 95% CI 0.24–1.81, P=0.415). Conversely, tonsillectomy was associated with an increased odds of HPV-positive BOT cancer (OR=2.46, 95% CI 1.22–4.95, P=0.012) and a weak and imprecise increase in odds of HPV-negative BOT cancer (OR=1.24, 95% CI 0.46–3.31, P=0.671). Tonsillectomy was also associated with a significantly increased odds of p16-positive BOT cancer (OR=2.24, 95% CI 1.16–4.35, P=0.017) and only a weak increase in odds of p16-negative BOT cancer (OR=1.21, 95% CI 0.43–3.36, P=0.716). Tonsillectomy was associated with decreased odds of p16-positive tonsil cancer (OR=0.14, 95% CI 0.07–0.31, P<0.001). A decrease of p16-negative tonsil cancer was also seen, but the OR had a very wide CI (OR=0.81, 95% CI 0.31–2.10, P=0.670). Finally, similar results were noted between previous tonsillectomy, HPV+/p16+ BOT cancer (OR=2.18, 95% CI 1.1–4.4), and HPV+/p16+ tonsil cancer (OR=0.16, 95% CI 0.08–0.37).

Table 4

Adjusted odds ratio for tonsillectomy and oropharyngeal subsite and HPV/p16 status compared with controlsa

	Base of tongue/HPV+			Base of tongue/HPV−			Tonsil/HPV+			Tonsil/HPV−
	OR (95% CI)	P	N (%)	OR (95% CI)	P	N (%)	OR (95% CI)	P	N (%)	OR (95% CI)	P	N (%)
Tonsil removed
No	Ref	–	17 (65.4%)	Ref	–	21 (72.4%)	Ref	–	99 (79.2%)	Ref	–	32 (82.1%)
Yes	2.46 (1.22–4.95)	0.012	9 (34.6%)	1.24 (0.46–3.31)	0.671	8 (27.6%)	0.17 (0.08–0.34)	<0.001	26 (20.8%)	0.66 (0.24–1.81)	0.415	7 (17.9%)

Abbreviations: CI=confidence interval; HPV=human papillomavirus; OR=odds ratio; Ref=reference value.

Adjusted for number of sexual partners, insurance, education, pack-years, alcohol, age, race, and gender.

Discussion

This population-based case–control study explores the association between previous tonsillectomy and odds of OPSCC. We found that previous tonsillectomy was associated with a significantly decreased odds of OPSCC overall that was driven by a sharp and expected decrease in tonsil cancer specifically. These data suggest that tonsillectomy results in a reduction of oropharyngeal lymphoid tissue susceptible to malignant transformation (Fakhry ). An unexpected finding from this analysis was the positive association between odds of BOT cancer and previous tonsillectomy. Although having a tonsillectomy is inversely associated with odds of tonsil cancer, our data suggest that tonsillectomy may be associated with an increased odds of BOT cancer. One possible explanation is lingual tonsillar hypertrophy in patients with a history of tonsillectomy, resulting in increased lymphoid tissue in the BOT. Enlargement of the lingual tonsils has been noted in up to one-third of paediatric patients undergoing adenotonsillectomy (Fricke ), and is a recognised cause of persistent sleep apnoea and airway obstruction in this population (Jesberg, 1956; Golding-Wood and Whittet, 1989; Cohle ; Jones and Cohle, 1993; Breitmeier ; Abdel-Aziz ; Friedman ). Our data suggest that the odds of BOT cancer is strongly increased in patients undergoing tonsillectomy as children (age <13 years) compared with adolescents and adults (age >13 years). The difference in odds of BOT cancer by age of tonsillectomy further supports the possible association between lingual tonsillar hypertrophy and increased susceptibility to BOT cancer. The most common indication for tonsillectomy in children is obstruction secondary to adenotonsillar hypertrophy (Smith and Pereira, 2007; Macfarlane ). In contrast, the most common indication for tonsillectomy in adults is chronic or recurrent tonsillitis (Hoddeson and Gourin, 2009).

An exploratory analysis of the association between tonsillectomy, tonsil, and BOT cancer, and HPV status yielded interesting results. When both subsite and HPV status were considered, we demonstrated that tonsillectomy was associated with a significantly decreased odds of HPV-positive tonsil cancer and a decreased but insignificant odds of HPV-negative tonsil cancer. The weaker association between tonsillectomy and HPV-negative tonsil cancer may result from susceptibility of nonlymphoid tissues of the tonsillar region (anterior tonsillar pillars, posterior tonsillar pillars, and tonsillar fossa) to HPV-negative, tobacco-associated cancer even in the setting of previous tonsillectomy. Ultimately, the decrease in odds of tonsil cancer in patients with a history of tonsillectomy is expected and logical. The association by HPV status should be explored further in larger studies. With respect of HPV-positive and HPV-negative BOT cancer, our data suggest that the association between tonsillectomy and an increased odds of BOT cancer is strongest for HPV-positive tumours. The reason for this difference by HPV status remains unclear and warrants further investigation, and may reflect a relatively small sample size of HPV-negative tumours.

Recently, the possibility of prophylactic tonsillectomy as a method of secondary OPSCC prevention has been considered (Chaturvedi, 2015; Fakhry ). Although we demonstrate a reduction in overall risk of OPSCC cancer associated with tonsillectomy, our study was not designed to address this question. It is important to consider that despite being a commonly performed surgery, the morbidity of tonsillectomy is not insignificant (Randall and Hoffer, 1998; Windfuhr , 2009). Furthermore, as demonstrated by Fakhry ), the relative rarity of OPSCC further precludes tonsillectomy from being an effective method of prevention. Vaccination against high-risk HPV types has the potential to serve as a safer and more effective method of primary prevention of OPSCC than tonsillectomy (D'Souza and Dempsey, 2011). Nonetheless, when considered along with other covariates such as sexual history, smoking status, and age, a history of tonsillectomy may play a role in risk stratification (Chaturvedi, 2015; Fakhry ).

This study has several limitations, many of which are inherent to the case–control study design. The use of self-reported tonsillectomy questionnaire data presents the possibility of recall bias and differential misclassification by disease status. However, recall of tonsillectomy would be very long for both the cases and the controls and at the time of recruitment. Because most tonsillectomies among cases and controls occurred at a very young age, medical record abstract is not feasible. Two previous studies have examined the accuracy of tonsillectomy recall, demonstrating sensitivity and specificity of recall were 92.7% and 77.5% in one study and 78.9% and 80.1% in the other, respectively (Krall ; Linet ). Using the false discovery and false omission rates from these studies, we conducted a sensitivity analysis (methods in Supplementary Material) and found that our estimates for tonsillectomy and tonsil cancer are very stable to changes in recall (Supplementary Table S2). However, for BOT cancer as differential recall between cases and controls widens, the estimates move closer to null and eventually crosses the null (Supplementary Table S1). It is unlikely that recall was that dramatically different between the cases and the controls. Another important limitation of this study is that tumour tissue was not available for all OPSCC samples in this cohort, limiting our analysis including HPV to a secondary, exploratory analysis. Tonsil cancer samples were more likely to be typed and this could potentially lead to selection bias affecting our analysis by HPV and p16 status. However, subjects with available HPV status and those without were similar with respect to demographics, age, smoking history, number of sexual partners, and stage at presentation, suggesting that HPV status would be similar between those typed and nontyped. Possible selection bias would not affect our overall results with respect to risk of tonsil vs BOT cancer, or our results incorporating age at tonsillectomy, as all cases were included in these analyses. Furthermore, our results involving HPV and p16 typing were stratified by subsite, thus diminishing the potential impact of any selection bias. A final limitation of this study is that some of the comparisons were based on small numbers and ORs were associated with wide CIs. Despite these limitations, this study offers important insight into the association between previous tonsillectomy and risk of OPSCC.

The major strengths of this study include a relatively large number of diverse patients and a population-based, case–control study design. The availability of detailed smoking, HPV tumour status, sexual history, socioeconomic status, and other demographic covariates, as well as a frequency-matched control group, add to the validity of our findings. Data on age of tonsillectomy allowed us to demonstrate that the majority of cases and controls underwent tonsillectomy as children and presumably for reasons other than suspected oropharyngeal cancer. A very small fraction of cases and controls underwent tonsillectomy within 10 years of OPSCC diagnosis or enrolment in CHANCE, respectively. When a sensitivity analysis was conducted excluding subjects with a history of tonsillectomy within 10 years of enrolment in CHANCE, there were no significant differences in the study findings.

In conclusion, this population-based case–control study demonstrates that previous tonsillectomy is associated with a decreased odds of tonsil cancer and an increased odds of BOT cancer. Although these finding do not necessarily support the role of tonsillectomy as primary prevention, tonsillectomy should be considered when evaluating risk for OPSCC.