Terhi Keltanen1, Anna-Mari Walta2, Satu Salonen2, Antti Sajantila2, Katarina Lindroos2. 1. Department of Forensic Medicine, University of Helsinki, P.O. Box 40, 00014, Helsinki, Finland. terhi.keltanen@helsinki.fi. 2. Department of Forensic Medicine, University of Helsinki, P.O. Box 40, 00014, Helsinki, Finland.
Abstract
PURPOSE: Renal markers may provide valuable information for cause of death (CoD) investigation when assessing the influence of impaired kidney function. A commonly used marker, creatinine (Cr), increases due to kidney injury and is known to be reasonably stable in postmortem (PM) samples. More sensitive markers are needed, as the increase of serum Cr level only occurs after relatively severe renal damage. We evaluated two markers, cystatin C and neutrophil gelatinase-associated lipocalin (NGAL), in addition to Cr. To the best of our knowledge this was the first study to investigate cystatin C and NGAL in a postmortem (PM) context. METHODS: Cr, cystatin C, and NGAL were measured from PM blood in 39 autopsy cases. NGAL was also measured from urine in 16 cases. Cystatin C and NGAL were analyzed using ELISA, Cr measurements were performed with Jaffe method. Correlations of these markers were evaluated. RESULTS AND CONCLUSIONS: Both, blood cystatin C and NGAL, levels showed significant correlation with Cr (p = 0.05 and p = 0.01, respectively). Cystatin C and NGAL in blood are promising markers for further studies with PM samples.
PURPOSE: Renal markers may provide valuable information for cause of death (CoD) investigation when assessing the influence of impaired kidney function. A commonly used marker, creatinine (Cr), increases due to kidney injury and is known to be reasonably stable in postmortem (PM) samples. More sensitive markers are needed, as the increase of serum Cr level only occurs after relatively severe renal damage. We evaluated two markers, cystatin C and neutrophil gelatinase-associated lipocalin (NGAL), in addition to Cr. To the best of our knowledge this was the first study to investigate cystatin C and NGAL in a postmortem (PM) context. METHODS:Cr, cystatin C, and NGAL were measured from PM blood in 39 autopsy cases. NGAL was also measured from urine in 16 cases. Cystatin C and NGAL were analyzed using ELISA, Cr measurements were performed with Jaffe method. Correlations of these markers were evaluated. RESULTS AND CONCLUSIONS: Both, blood cystatin C and NGAL, levels showed significant correlation with Cr (p = 0.05 and p = 0.01, respectively). Cystatin C and NGAL in blood are promising markers for further studies with PM samples.
Authors: Vishal S Vaidya; Sushrut S Waikar; Michael A Ferguson; Fitz B Collings; Kelsey Sunderland; Costas Gioules; Gary Bradwin; Roland Matsouaka; Rebecca A Betensky; Gary C Curhan; Joseph V Bonventre Journal: Clin Transl Sci Date: 2008-12 Impact factor: 4.689
Authors: C R Parikh; J Mishra; H Thiessen-Philbrook; B Dursun; Q Ma; C Kelly; C Dent; P Devarajan; C L Edelstein Journal: Kidney Int Date: 2006-05-17 Impact factor: 10.612
Authors: Stefan Herget-Rosenthal; Günter Marggraf; Johannes Hüsing; Frauke Göring; Frank Pietruck; Onno Janssen; Thomas Philipp; Andreas Kribben Journal: Kidney Int Date: 2004-09 Impact factor: 10.612
Authors: Peter A McCullough; Andrew D Shaw; Michael Haase; Josee Bouchard; Sushrut S Waikar; Edward D Siew; Patrick T Murray; Ravindra L Mehta; Claudio Ronco Journal: Contrib Nephrol Date: 2013-05-13 Impact factor: 1.580