Jeyanthi Suppiah1, T S Saraswathy2, K Amry2, Apandi Yusof2, Zainah Saat2. 1. Virology Unit, Institute for Medical Research, Kuala Lumpur, Malaysia. Electronic address: jeyanthi@imr.gov.my. 2. Virology Unit, Institute for Medical Research, Kuala Lumpur, Malaysia.
Abstract
OBJECTIVE: To identify the circulating serotypes of human echovirus in Malaysia from 2002 to 2013. METHODS: A total of 31 retrospective samples from non-polio acute flacid paralysis, hand-food-and-mouth disease, viral meningitis and enterovirus cases were subjected to amplification of partial VP1 gene by RT-PCR. RESULTS: Sequencing and phylogenetic analysis of the partial sequences identified presence of human echovirus and human coxsackie viruses. It was found that echovirus 11 was the commonly circulating serotype followed by echovirus 6, echovirus 7, echovirus 3, echovirus 9, echovirus 30 and echovirus 1 in decreasing order. Additionally two types of human coxsackie virus isolates were detected which were coxsackie A24 and B3. CONCLUSIONS: From the findings, there is a possibility that echovirus 11 is the predominant serotype among Malaysian patients with echovirus infection. However, a larger sample size will yield a more confident result to support this evidence.
OBJECTIVE: To identify the circulating serotypes of human echovirus in Malaysia from 2002 to 2013. METHODS: A total of 31 retrospective samples from non-polio acute flacid paralysis, hand-food-and-mouth disease, viral meningitis and enterovirus cases were subjected to amplification of partial VP1 gene by RT-PCR. RESULTS: Sequencing and phylogenetic analysis of the partial sequences identified presence of human echovirus and human coxsackie viruses. It was found that echovirus 11 was the commonly circulating serotype followed by echovirus 6, echovirus 7, echovirus 3, echovirus 9, echovirus 30 and echovirus 1 in decreasing order. Additionally two types of human coxsackie virus isolates were detected which were coxsackie A24 and B3. CONCLUSIONS: From the findings, there is a possibility that echovirus 11 is the predominant serotype among Malaysian patients with echovirus infection. However, a larger sample size will yield a more confident result to support this evidence.
Authors: Endrya do Socorro Fôro Ramos; Ulisses Alves Rosa; Geovani de Oliveira Ribeiro; Fabiola Villanova; Flávio Augusto de Pádua Milagres; Rafael Brustulin; Vanessa Dos Santos Morais; Mayara Bertanhe; Roberta Marcatti; Emerson Luiz Lima Araújo; Steven S Witkin; Eric Delwart; Adriana Luchs; Antonio Charlys da Costa; Élcio Leal Journal: Viruses Date: 2021-03-31 Impact factor: 5.048