Zuzanna Gronkiewicz1, Wojciech Kukwa1, Leszek Krolicki2, Agata Cyran-Chlebicka3, Dariusz Pawlak4, Czeslaw Stankiewicz5, Antoni Krzeski1, Barbara Górnicka3, Dominika Wolosz3, Jolanta Kunikowska2. 1. Department of Otorhinolaryngology, Faculty of Medicine & Dentistry, Medical University of Warsaw, 19/25 Stepinska Street, 00-739 Warsaw, Poland. 2. Nuclear Medicine Department, Medical University of Warsaw, 1a Banacha Street, 02-097 Warsaw, Poland. 3. Department of Pathology, Medical University of Warsaw, 7 Pawinskiego Street, 02-106 Warsaw, Poland. 4. Radioisotope Centre POLATOM, National Centre for Nuclear Research, Otwock, Poland. 5. Department of Otolaryngology, Medical University of Gdansk, 7 Debinki Street, 80-952 Gdansk, Poland.
Abstract
BACKGROUND: As somatostatin receptors (SSTRs) may be overexpressed in rapidly growing vessels, the aim of this study was the analysis of in vivo and in vitro SSTR2A expression in juvenile angiofibroma (JA). MATERIAL & METHODS: A group of six male adolescents with a diagnosis of primary, recurrent/residual JA was enrolled in the study. All patients underwent (68)Ga-DOTATATE PET/computed tomography (CT) followed by immunohistochemical staining for SSTR expression. RESULTS: (68)Ga-DOTATATE PET/CT showed accumulation in areas matching the pathologic tissue in the nasopharynx of all patients studied with SUVmax of 5.1 ± 0.9 (ranging from 3.6 to 6.4). In all cases, the immunohistochemical examination showed a presence of SSTR2A with a high staining index. CONCLUSION: In vitro SSTR2A cytoplasm expression was found to be high in all tumor specimens. However, the uptake of (68)Ga-DOTATATE was weak in the PET/CT studies. We postulate that the intracellular localization of the SSTR2A in JA may cause this discrepancy.
BACKGROUND: As somatostatin receptors (SSTRs) may be overexpressed in rapidly growing vessels, the aim of this study was the analysis of in vivo and in vitro SSTR2A expression in juvenile angiofibroma (JA). MATERIAL & METHODS: A group of six male adolescents with a diagnosis of primary, recurrent/residual JA was enrolled in the study. All patients underwent (68)Ga-DOTATATE PET/computed tomography (CT) followed by immunohistochemical staining for SSTR expression. RESULTS: (68)Ga-DOTATATE PET/CT showed accumulation in areas matching the pathologic tissue in the nasopharynx of all patients studied with SUVmax of 5.1 ± 0.9 (ranging from 3.6 to 6.4). In all cases, the immunohistochemical examination showed a presence of SSTR2A with a high staining index. CONCLUSION: In vitro SSTR2A cytoplasm expression was found to be high in all tumor specimens. However, the uptake of (68)Ga-DOTATATE was weak in the PET/CT studies. We postulate that the intracellular localization of the SSTR2A in JA may cause this discrepancy.
Entities:
Keywords:
68Ga-DOTATATE PET/CT; head and neck; imaging; immunohistochemistry; juvenile angiofibroma; somatostatin receptor