Literature DB >> 26971637

MALDI-TOF mass spectrometry for differentiation between Streptococcus pneumoniae and Streptococcus pseudopneumoniae.

Joffrey van Prehn1, Suzanne Q van Veen2, Jacqueline J G Schelfaut3, Els Wessels3.   

Abstract

We compared the Vitek MS and Microflex MALDI-TOF mass spectrometry platform for species differentiation within the Streptococcus mitis group with PCR assays targeted at lytA, Spn9802, and recA as reference standard. The Vitek MS correctly identified 10/11 Streptococcus pneumoniae, 13/13 Streptococcus pseudopneumoniae, and 12/13 S. mitis/oralis. The Microflex correctly identified 9/11 S. pneumoniae, 0/13 S. pseudopneumoniae, and 13/13 S. mitis/oralis. MALDI-TOF is a powerful tool for species determination within the mitis group. Diagnostic accuracy varies depending on platform and database used.
Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved.

Entities:  

Keywords:  Diagnostic accuracy; MALDI-TOF; Mass spectrometry; Mitis group; PCR; Streptococcus

Mesh：

Year:  2016        PMID： 26971637     DOI： 10.1016/j.diagmicrobio.2016.01.012

Source DB:  PubMed          Journal:  Diagn Microbiol Infect Dis        ISSN： 0732-8893            Impact factor:   2.803


  8 in total

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4.  Identification and molecular epidemiology of routinely determined Streptococcus pneumoniae with negative Quellung reaction results.

Authors:  Ju Jia; Wei Shi; Fang Dong; Qingying Meng; Lin Yuan; Changhui Chen; Kaihu Yao
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6.  Differentiating between Enterococcusfaecium and Enterococcuslactis by Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry.

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Journal:  Foods       Date:  2022-04-05

7.  Assessment of a novel bile solubility test and MALDI-TOF for the differentiation of Streptococcus pneumoniae from other mitis group streptococci.

Authors:  Hans-Christian Slotved; Richard R Facklam; Kurt Fuursted
Journal:  Sci Rep       Date:  2017-08-02       Impact factor: 4.379

8.  Identification of closely related Ixodes species by protein profiling with MALDI-TOF mass spectrometry.

Authors:  Pierre H Boyer; Lionel Almeras; Olivier Plantard; Antoine Grillon; Émilie Talagrand-Reboul; Karen McCoy; Benoît Jaulhac; Nathalie Boulanger
Journal:  PLoS One       Date:  2019-10-17       Impact factor: 3.240
  8 in total

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