Chao Meng1, Liangjuan Ma2, Li Niu3, Xiaoguang Cui1, Jinfeng Liu1, Jiyu Kang4, Rongfang Liu1, Jingchun Xing1, Changlin Jiang5, Huacheng Zhou6. 1. Department of Anesthesiology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Hei Longjiang Province 150001, China; The Hei Longjiang Province Key Lab of Research on Anesthesiology and Critical Care Medicine, Harbin, Hei Longjiang Province 150001, China. 2. Department of Dermatology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Hei Longjiang Province 150001, China. 3. Department of Anesthesiology, The 211 Hospital of the Chinese People's Liberation Army, Harbin, Hei Longjiang Province 150001, China. 4. Department of Anesthesiology, The Fourth Affiliated Hospital of Harbin Medical University, Harbin, Hei Longjiang Province 150001, China. 5. Department of Anesthesiology, The General Hospital of Daqing Oilfield, Daqing, Hei Longjiang Province 163000, China. 6. Department of Anesthesiology, The Fourth Affiliated Hospital of Harbin Medical University, Harbin, Hei Longjiang Province 150001, China. Electronic address: zhouhuacheng@163.com.
Abstract
AIMS: Lung ischemia-reperfusion injury (IRI) may be attenuated through carbon monoxide (CO)'s anti-inflammatory effect or hydrogen (H2)'s anti-oxidant effect. In this study, the effects of lung inflation with CO, H2, or both during the cold ischemia phase on graft function were observed. MATERIALS AND METHODS: Rat donor lungs, inflated with 40% oxygen (control group), 500ppm CO (CO group), 3% H2 (H2 group) or 500ppm CO+3% H2 (COH group), were kept at 4°C for 180min. After transplantation, the recipients' artery blood gas and pressure-volume (P-V) curves were analyzed. The inflammatory response, oxidative stress and apoptosis in the recipients were assessed at 180min after reperfusion. KEY FINDINGS: Oxygenation in the CO and H2 groups were improved compared with the control group. The CO and H2 groups also exhibited significantly improved P-V curves, reduced lung injury, and decreased inflammatory response, malonaldehyde content, and cell apoptosis in the grafts. Furthermore, the COH group experienced enhanced improvements in oxygenation, P-V curves, inflammatory response, lipid peroxidation, and graft apoptosis compared to the CO and H2 groups. SIGNIFICANCE: Lung inflation with CO or H2 protected against IRI via anti-inflammatory, anti-oxidant and anti-apoptotic mechanisms in a model of lung transplantation in rats, which was enhanced by combined treatment with CO and H2.
AIMS: Lung ischemia-reperfusion injury (IRI) may be attenuated through carbon monoxide (CO)'s anti-inflammatory effect or hydrogen (H2)'s anti-oxidant effect. In this study, the effects of lung inflation with CO, H2, or both during the cold ischemia phase on graft function were observed. MATERIALS AND METHODS:Ratdonor lungs, inflated with 40% oxygen (control group), 500ppm CO (CO group), 3% H2 (H2 group) or 500ppm CO+3% H2 (COH group), were kept at 4°C for 180min. After transplantation, the recipients' artery blood gas and pressure-volume (P-V) curves were analyzed. The inflammatory response, oxidative stress and apoptosis in the recipients were assessed at 180min after reperfusion. KEY FINDINGS: Oxygenation in the CO and H2 groups were improved compared with the control group. The CO and H2 groups also exhibited significantly improved P-V curves, reduced lung injury, and decreased inflammatory response, malonaldehyde content, and cell apoptosis in the grafts. Furthermore, the COH group experienced enhanced improvements in oxygenation, P-V curves, inflammatory response, lipid peroxidation, and graft apoptosis compared to the CO and H2 groups. SIGNIFICANCE: Lung inflation with CO or H2 protected against IRI via anti-inflammatory, anti-oxidant and anti-apoptotic mechanisms in a model of lung transplantation in rats, which was enhanced by combined treatment with CO and H2.