Sajjad Ahmad1, Farhat Ullah1, Muhammad Ayaz1, Anwar Zeb1, Farman Ullah2, Abdul Sadiq3. 1. Department of Pharmacy, University of Malakand, Chakdara, Dir (L), Khyber-Pakhtunkhwa (KPK), 18000, Pakistan. 2. Department of Pharmacy, Kohat University of Science and Technology, Kohat, Khyber-Pakhtunkhwa (KPK), 26000, Pakistan. 3. Department of Pharmacy, University of Malakand, Chakdara, Dir (L), Khyber-Pakhtunkhwa (KPK), 18000, Pakistan. sadiquom@yahoo.com.
Abstract
BACKGROUND: Cancer, being the foremost challenge of the modern era and the focus of world-class investigators, gargantuan research is in progress worldwide to explore novel therapeutic for its management. The exploitation of natural sources has been proven to be an excellent approach to treat or minify the excessive angiogenesis and proliferation of cells. Similarly, based the ethnomedicinal uses and literature survey, the current study is designed to explore the anti-tumor and anti-angiogenic potentials of Rumex hastatus. Anti-tumor and anti-angiogenic activities were carried out using potato-disc model and chorioallantoic membrane (CAM) assay respectively. Moreover, R. hastatus was also assessed for antibacterial activity against Agrobacterium tumefaciens (tumor causing bacterial strain). The positive controls used in anti-tumor, anti-angiogenic and antibacterial activities were vincristine sulphate, dexamethasone and cefotaxime respectively. RESULTS: The crude saponins (Rh.Sp), methanolic extract (Rh.Cr) and other solvent extracts like n-hexane (Rh.Hex), chloroform (Rh.Chf), ethylacetate (Rh.EtAc) and aqueous fraction (Rh.Aq) exhibited notable anti-tumor and anti-angiogenic activities. In potato tumor assay, the chloroform and saponin fractions were observed to be the most effective showing 86.7 and 93.3 % tumor inhibition at 1000 µg/ml with IC50 values 31.6 and 18.1 µg/ml respectively. Similarly, these two samples i.e., chloroform and saponins also excelled among the entire test samples in anti-angiogenic evaluation exhibiting 81.6 % (IC50 = 17.9 µg/ml) and 78.9 % (IC50 = 64.9 µg/ml) at 1000 µg/ml respectively. In contrast, the antibacterial investigations revealed a negligible potential against A. tumefaciens. CONCLUSION: Based on our results we can claim that R. hastatus possesses both anti-tumor and anti-angiogenic potentials. In all of the solvent fractions, Rh.Chf and Rh.Sp were most effective against tumor and angiogenesis while having negligible activity against A. tumefaciens. It can be concluded that Rh.Chf and Rh.Sp might be potential targets in the isolation of natural product having anti-neoplastic action.
BACKGROUND:Cancer, being the foremost challenge of the modern era and the focus of world-class investigators, gargantuan research is in progress worldwide to explore novel therapeutic for its management. The exploitation of natural sources has been proven to be an excellent approach to treat or minify the excessive angiogenesis and proliferation of cells. Similarly, based the ethnomedicinal uses and literature survey, the current study is designed to explore the anti-tumor and anti-angiogenic potentials of Rumex hastatus. Anti-tumor and anti-angiogenic activities were carried out using potato-disc model and chorioallantoic membrane (CAM) assay respectively. Moreover, R. hastatus was also assessed for antibacterial activity against Agrobacterium tumefaciens (tumor causing bacterial strain). The positive controls used in anti-tumor, anti-angiogenic and antibacterial activities were vincristine sulphate, dexamethasone and cefotaxime respectively. RESULTS: The crude saponins (Rh.Sp), methanolic extract (Rh.Cr) and other solvent extracts like n-hexane (Rh.Hex), chloroform (Rh.Chf), ethylacetate (Rh.EtAc) and aqueous fraction (Rh.Aq) exhibited notable anti-tumor and anti-angiogenic activities. In potatotumor assay, the chloroform and saponin fractions were observed to be the most effective showing 86.7 and 93.3 % tumor inhibition at 1000 µg/ml with IC50 values 31.6 and 18.1 µg/ml respectively. Similarly, these two samples i.e., chloroform and saponins also excelled among the entire test samples in anti-angiogenic evaluation exhibiting 81.6 % (IC50 = 17.9 µg/ml) and 78.9 % (IC50 = 64.9 µg/ml) at 1000 µg/ml respectively. In contrast, the antibacterial investigations revealed a negligible potential against A. tumefaciens. CONCLUSION: Based on our results we can claim that R. hastatus possesses both anti-tumor and anti-angiogenic potentials. In all of the solvent fractions, Rh.Chf and Rh.Sp were most effective against tumor and angiogenesis while having negligible activity against A. tumefaciens. It can be concluded that Rh.Chf and Rh.Sp might be potential targets in the isolation of natural product having anti-neoplastic action.
Authors: Muhammad Ayaz; Muhammad Junaid; Jawad Ahmed; Farhat Ullah; Abdul Sadiq; Sajjad Ahmad; Muhammad Imran Journal: BMC Complement Altern Med Date: 2014-05-03 Impact factor: 3.659
Authors: Sajid Hussain; Farhat Ullah; Muhammad Ayaz; Syed Adnan Ali Shah; Azhar-Ul-Haq Ali Shah; Syed Majid Shah; Abdul Wadood; Waqar Aman; Riaz Ullah; Abdelaaty A Shahat; Fahd A Nasr Journal: Drug Des Devel Ther Date: 2019-12-10 Impact factor: 4.162
Authors: Fawad Mahmood; Muhammad S Jan; Sajjad Ahmad; Umer Rashid; Muhammad Ayaz; Farhat Ullah; Fida Hussain; Ashfaq Ahmad; Arif-Ullah Khan; Muhammad Aasim; Abdul Sadiq Journal: Front Chem Date: 2017-12-12 Impact factor: 5.221
Authors: Nausheen Nazir; Mohammad Nisar; Sajjad Ahmad; Syed Fazal Wadood; Tour Jan; Muhammad Zahoor; Manzoor Ahmad; Abid Ullah Journal: Environ Sci Pollut Res Int Date: 2019-12-30 Impact factor: 5.190
Authors: Muhammad Ayaz; Muhammad Junaid; Farhat Ullah; Abdul Sadiq; Fazal Subhan; Mir Azam Khan; Waqar Ahmad; Gowhar Ali; Muhammad Imran; Sajjad Ahmad Journal: Front Pharmacol Date: 2016-03-31 Impact factor: 5.810