Baran Şen1, Serkan Güler1, Berivan Çeçen2, Erdem Kumtepe2, Alper Bağrıyanık3, Sermin Özkal4, M Ali Özcan5, Hayri Özsan5, Namık Şanlı5, M Hasan Tatari1. 1. Department of Orthopaedics and Traumatology, Dokuz Eylül University Faculty of Medicine, İzmir, Turkey. 2. Department of Biomechanics, Dokuz Eylül University Institute of Health Science, İzmir, Turkey. 3. Department of Histology and Embriology, Dokuz Eylül University Faculty of Medicine, İzmir, Turkey. 4. Department of Pathology, Dokuz Eylül University Faculty of Medicine, İzmir, Turkey. 5. Department of Hematology, Dokuz Eylül University Faculty of Medicine, İzmir, Turkey.
Abstract
BACKGROUND: Achilles tendon ruptures are characterized by a long recovery period, high re-rupture rate and late return to work. To overcome these difficulties and augment tendon repair, many agents have been used. AIMS: To determine the effect of autologous platelet rich plasma (PRP) in the treatment of Achilles tendon ruptures in rabbits. STUDY DESIGN: Animal experimentation. METHODS: The study included 14 New Zealand albino rabbits that were divided randomly into 2 groups, A and B, each containing seven rabbits. On day zero, all 28 Achilles tendons were tenotomized and repaired. In group A, the tendons were injected with PRP post-surgery, whereas those in group B were left untreated. On day 28, the right tendons in both groups were examined histopathologically via both light and electron microscopy, and the left tendons were subjected to biomechanical testing. RESULTS: The histological and biomechanical findings in both light and electron microscopy in group A were better than those in group B, but the difference was not significant. According to Tang's scale, the mean value in Group A was 3.57, while it was 3.0 in Group B. The mean value of Group A for the length of collagen bands was 48.09 nm while the mean value of Group B was 46.58 nm (p=0.406). In biomechanical tests, although stiffness values were higher in group A, the difference between groups was not significant. In addition, maximum load values did not differ between groups A and B. CONCLUSION: PRP had no effect on the healing process 28 days post-Achilles tendon rupture.
BACKGROUND:Achilles tendon ruptures are characterized by a long recovery period, high re-rupture rate and late return to work. To overcome these difficulties and augment tendon repair, many agents have been used. AIMS: To determine the effect of autologous platelet rich plasma (PRP) in the treatment of Achilles tendon ruptures in rabbits. STUDY DESIGN: Animal experimentation. METHODS: The study included 14 New Zealand albino rabbits that were divided randomly into 2 groups, A and B, each containing seven rabbits. On day zero, all 28 Achilles tendons were tenotomized and repaired. In group A, the tendons were injected with PRP post-surgery, whereas those in group B were left untreated. On day 28, the right tendons in both groups were examined histopathologically via both light and electron microscopy, and the left tendons were subjected to biomechanical testing. RESULTS: The histological and biomechanical findings in both light and electron microscopy in group A were better than those in group B, but the difference was not significant. According to Tang's scale, the mean value in Group A was 3.57, while it was 3.0 in Group B. The mean value of Group A for the length of collagen bands was 48.09 nm while the mean value of Group B was 46.58 nm (p=0.406). In biomechanical tests, although stiffness values were higher in group A, the difference between groups was not significant. In addition, maximum load values did not differ between groups A and B. CONCLUSION: PRP had no effect on the healing process 28 days post-Achilles tendon rupture.
Authors: Tiffany L Sarrafian; Hali Wang; Eileen S Hackett; Jian Q Yao; Mei-Shu Shih; Heather L Ramsay; A Simon Turner Journal: J Foot Ankle Surg Date: 2010-02-04 Impact factor: 1.286
Authors: Thorsten Schepull; Joanna Kvist; Hanna Norrman; Marie Trinks; Gösta Berlin; Per Aspenberg Journal: Am J Sports Med Date: 2010-11-03 Impact factor: 6.202
Authors: Angelo De Carli; Riccardo Maria Lanzetti; Alessandro Ciompi; Domenico Lupariello; Antonio Vadalà; Giuseppe Argento; Andrea Ferretti; M C Vulpiani; M Vetrano Journal: Knee Surg Sports Traumatol Arthrosc Date: 2015-03-22 Impact factor: 4.342
Authors: Robert J de Vos; Adam Weir; Hans T M van Schie; Sita M A Bierma-Zeinstra; Jan A N Verhaar; Harrie Weinans; Johannes L Tol Journal: JAMA Date: 2010-01-13 Impact factor: 56.272
Authors: Mehmet Murat Bala; Abdullah Alper Şahin; Mehmet Boz; Yasin Durukan; Tülin Fırat; Murat Pakdil; Kutay Engin Özturan Journal: Med Sci Monit Date: 2021-09-06